Catalog No: OPPA00486 (Formerly GWB-70275C)
Size:5UG
Price: $75.00
SKU
OPPA00486
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/Manuals | Printable datasheet for OPPA00486 |
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Predicted Species Reactivity | Human |
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Product Format | Lyophilized after extensive dialysis against 2mM sodium phosphate buffer pH 7.4 +/- 0.1 Physical Appearance: Sterile filtered white powder |
Host | E. Coli |
:: | Protein Content: GM-CSF quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.963 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GEN computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard. |
Reconstitution and Storage | It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution GMCSF should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles. |
Purification | Purified by proprietary chromatographic techniques |
Purity | Greater than 98.0% as determined by: a) Analysis by RP-HPLC b) Analysis by SDS-PAGE |
Biological Activity | The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.1 ng/ml, corresponding to a Specific Activity of 11.1x106 IU/mg. |
Peptide Sequence | The sequence of the first five N - terminal amino acids was determined and was found to be Ala - Pro - Ala - Arg - Ser. N-terminal methionine has been completely removed enzymatically. |
Gene Symbol | GM CSF |
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Alias Symbols | CSF, GMCSF |
NCBI Gene Id | 1437 |
Protein Name | Granulocyte-macrophage colony-stimulating factor |
Description of Target | Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was first characterized as a growth factor that supports the in-vitro colony formation of granulocytes-macrophages progenitor cells. It is a pleiotropic cytokine and a member of a family of endogenous cytokines of the hematopoietic system. GM-CSF is produced as a response to immune or inflammatory stimuli by activated cells of the hematopoietic system such as T cells, B cells, macrophages, mast cells and also fibroblasts and alveolar epithelial cells. It plays an important role in regulating the proliferation, differentiation, survival and activation of hematopoietic cells such as granulocytes and monocytes ,neutrophiles, basophiles and eosonophoiles, erythroid cells, megakaryocytes and T cells. Human and mouse GM-CSF have about 56% homology and are species specific. Human GM-CSF is not active on mouse cells and vice versa. It is active on canine and feline cells. GMCSF is 144 amino acids, 22kDa glycoprotein. It is composed of four bundles alpha helices. Its receptor is heterodimers with a ligand-specific alpha subunit and a betac subunit that is shared with the interleukin IL-3 and IL-5 receptors. This unusual form of receptor assembly likely applies also to IL-3 and IL-5 receptors. Cross-linking the two receptor subunits is required for receptor activation and signaling . GMCSF has been shown to be involved in maturation, mobilization and antigen presentation of myeloid dentritic cells (DCs) in-vivo or ex-vivo. This function promotes Th1 immune responses, cytotoxcity, anti-angiogenesis as well as allergic inflammation, and the development of autoimmunity. Therefore GMCSF can be used in immunotherapy for the treatment of immune suppressed and immune-compromised patients as well as in veterinary medicine for the same purpose. GM-CSF is also important in regulation of embryo development and pregnancy and specifically in embryo implantation and subsequent development . |
Uniprot ID | P04141 |
Protein Accession # | NP_000749.2 |
Protein Size (# AA) | Recombinant |
Molecular Weight | 14 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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