Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OPPA00486 (Formerly GWB-70275C)
Size:5UG
Price: $75.00
SKU
OPPA00486
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

GM CSF Protein (OPPA00486)

Datasheets/ManualsPrintable datasheet for OPPA00486
Product Info
Predicted Species ReactivityHuman
Product FormatLyophilized after extensive dialysis against 2mM sodium phosphate buffer pH 7.4 +/- 0.1
Physical Appearance: Sterile filtered white powder
HostE. Coli
::Protein Content: GM-CSF quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.963 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GEN computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard.
Reconstitution and StorageIt is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution GMCSF should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
PurificationPurified by proprietary chromatographic techniques
PurityGreater than 98.0% as determined by:
a) Analysis by RP-HPLC
b) Analysis by SDS-PAGE
Biological ActivityThe ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.1 ng/ml, corresponding to a Specific Activity of 11.1x106 IU/mg.
Peptide SequenceThe sequence of the first five N - terminal amino acids was determined and was found to be Ala - Pro - Ala - Arg - Ser. N-terminal methionine has been completely removed enzymatically.
Gene SymbolGM CSF
Alias SymbolsCSF, GMCSF
NCBI Gene Id1437
Protein NameGranulocyte-macrophage colony-stimulating factor
Description of TargetGranulocyte Macrophage Colony Stimulating Factor (GM-CSF) was first characterized as a growth factor that supports the in-vitro colony formation of granulocytes-macrophages progenitor cells. It is a pleiotropic cytokine and a member of a family of endogenous cytokines of the hematopoietic system. GM-CSF is produced as a response to immune or inflammatory stimuli by activated cells of the hematopoietic system such as T cells, B cells, macrophages, mast cells and also fibroblasts and alveolar epithelial cells. It plays an important role in regulating the proliferation, differentiation, survival and activation of hematopoietic cells such as granulocytes and monocytes ,neutrophiles, basophiles and eosonophoiles, erythroid cells, megakaryocytes and T cells. Human and mouse GM-CSF have about 56% homology and are species specific. Human GM-CSF is not active on mouse cells and vice versa. It is active on canine and feline cells. GMCSF is 144 amino acids, 22kDa glycoprotein. It is composed of four bundles alpha helices. Its receptor is heterodimers with a ligand-specific alpha subunit and a betac subunit that is shared with the interleukin IL-3 and IL-5 receptors. This unusual form of receptor assembly likely applies also to IL-3 and IL-5 receptors. Cross-linking the two receptor subunits is required for receptor activation and signaling . GMCSF has been shown to be involved in maturation, mobilization and antigen presentation of myeloid dentritic cells (DCs) in-vivo or ex-vivo. This function promotes Th1 immune responses, cytotoxcity, anti-angiogenesis as well as allergic inflammation, and the development of autoimmunity. Therefore GMCSF can be used in immunotherapy for the treatment of immune suppressed and immune-compromised patients as well as in veterinary medicine for the same purpose. GM-CSF is also important in regulation of embryo development and pregnancy and specifically in embryo implantation and subsequent development .
Uniprot IDP04141
Protein Accession #NP_000749.2
Protein Size (# AA)Recombinant
Molecular Weight14 kDa
Write Your Own Review
You're reviewing:GM CSF Protein (OPPA00486)
Your Rating