GM CSF K9 Protein (OPPA02453)

Catalog No: OPPA02453 (Formerly GWB-BSP036)

Size:2UG

Product Info

• Predicted Species Reactivity: Dog
• Product Format: GMCSF was lyophilized after extensive dialysis against 1x PBS pH= 7.4. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Additional Information: Biological Activity: The ED50 as calculated by the dose-dependant stimulation of the proliferation of human TF1 erythroleukemic cells is typically 1-4 ng/ml.
  Product Introduction: GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
  Product Description: GMCSF k9 Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids and having a molecular mass of 14.2 kDa. GM-CSF is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Reconstitution: It is recommended to reconstitute the lyophilized GMCSF in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
  Storage:  Lyophilized GMCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution GMCSF should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purity: Greater than 96.0% as determined by: 1. Analysis by RP-HPLC 2. Analysis by SDS-PAGE.
• Peptide Sequence: APTRSPTLVT RPSQHVDAIQ EALSLLNNSN DVTAVMNKAV KVVSEVFDPEGPTCLETRLQ LYKEGLQGSL TSLKNPLTMM ANHYKQHCPP TPESPCATQNINFKSFKENL KDFLFNIPFD CWKPVKK.

Target Info

• Gene Symbol: GMCSF k9
• Alias Symbols: GM-CSF
• NCBI Gene Id: 403923
• Protein Name: Granulocyte-macrophage colony-stimulating factor
• Description of Target: Recombinant Canine Granulocyte Macrophage-Colony Stimulating Factor
• Uniprot ID: P48749
• Protein Accession #: NP_001003245.1
• Protein Size (# AA): Recombinant