GM-CSF ELISA Kit (Human) : 96 Wells (OKAG00019)

Catalog#: OKAG00019

• Size: 96 Wells

• Predicted Species Reactivity: Human
• Product Format: 1 x 96-Well Plate or 12 x 8-Well Strips
• Application: ELISA
• Reconstitution and Storage: Store as indicated in product manual.
• Gene Symbol: CSF2
• Official Gene Full Name: colony stimulating factor 2
• Alias Symbols: Colony-stimulating factor, CSF, GMCSF, GM-CSF, Granulocyte-macrophage colony-stimulating factor, MGC131935, MGC138897, Molgramostin, Sargramostim
• NCBI Gene Id: 1437
• Protein Name: Granulocyte-macrophage colony-stimulating factor
• Description of Target: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
• Swissprot Id: P04141
• Protein Accession #: NP_000749.2
• Nucleotide Accession #: NM_000758.3
• Sample Type: Cell lysates, sera and plasma
• Sensitivity: 32 pg/mL
• Kit Range: 32-1,000 pg/mL
• Kit Principle: The Aviva Human G-CSF ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hG-CSF concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human G-CSF cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration
• Kit Component:

  Components	Amount
  Capture Antibody Coated Microplate	96 Wells (12 x 8)
  Ready-to-Use Streptavidin-HRP	12 mL
  Ready-to-Use Substrate	12 mL
  Stop Solution	12 mL
  Wash Buffer (15x)	50 mL
  Protein Standard Diluent	12 mL
  Sample Diluent	12 mL
  Detection Antibody Diluent	12 mL
  Biotin-Labeled Detection Antibody	Lyophilized
  Protein Standard	Lyophilized (100 ng)

• Kit Detection Method: Colorimetric, OD450 nm
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express CSF2.
• Datasheets/Manuals: Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
• Specificity: The Human GM-CSF ELISA is capable of recognizing both recombinant and naturally produced Human GM-CSF proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
  • Human: SCF, M-CSF, TPO, G-CSF, IL-5
  • Murine: G-CSF, GM-CSF, M-CSF
  • Rat: GM-CSF
• Assay Info: Quantitative Colorimentric Sandwich ELISA

Protocol:

Tips Information: