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GM-CSF ELISA Kit (Human) : 96 Wells (OKAG00019)

96 Wells
$479.00
In Stock
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Predicted Species Reactivity:
Human
Product Format:
1 x 96-Well Plate or 12 x 8-Well Strips
Application:
ELISA
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
CSF2
Official Gene Full Name:
colony stimulating factor 2
Alias Symbols:
Colony-stimulating factor, CSF, GMCSF, GM-CSF, Granulocyte-macrophage colony-stimulating factor, MGC131935, MGC138897, Molgramostin, Sargramostim
NCBI Gene Id:
1437
Protein Name:
Granulocyte-macrophage colony-stimulating factor
Description of Target:
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
Swissprot Id:
P04141
Protein Accession #:
NP_000749.2
Nucleotide Accession #:
NM_000758.3
Sample Type:
Cell lysates, sera and plasma
Sensitivity:
32 pg/mL
Kit Range:
32-1,000 pg/mL
Kit Principle:
The Aviva Human G-CSF ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hG-CSF concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human G-CSF cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration
Kit Component:
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (100 ng)
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CSF2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CSF2.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
The Human GM-CSF ELISA is capable of recognizing both recombinant and naturally produced Human GM-CSF proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: SCF, M-CSF, TPO, G-CSF, IL-5
• Murine: G-CSF, GM-CSF, M-CSF
• Rat: GM-CSF
Assay Info:
Quantitative Colorimentric Sandwich ELISA

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