Catalog No: OKEH02622
Size:96T
Price: $600.00
SKU
OKEH02622
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Glutathione ELISA Kit (OKEH02622) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Glutathione antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Glutathione and incubated. The Glutathione found in the sample or standards competes with the biotinylated Glutathione for limited binding sites on the immobilized anti-Glutathione antibody. Excess unbound biotinylated Glutathione and sample or standard Glutathione is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Glutathione captured in the well and inversely proportional to the amount of Glutathione which was contained in the sample or standard.
ELISA Kit Range1.25-80ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 7.8% (n = 20)
Mean Inter-assay CV%: < 9.6% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Glutathione Microplate96 Wells (12 x 8 Well strips)
Glutathione Lyophilized Standard2 x 80 ng
Sample Diluent1 x 20 mL
100X Glutathione-Biotin Complex1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: GSH
Target Name: Glutathione
::Pubchem: 124886
::Chemical formula: C10H17N3O6S
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.625 ng/mL
SpecificityNatural and recombinant General Glutathione
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsGSH, Glutathione, GSH
Protein NameGlutathione
Description of TargetGlutathione (GSH) is a tripeptide with a gamma peptide linkage between the amine group of cysteine (which is attached by normal peptide linkage to a glycine) and the carboxyl group of the glutamate side-chain. It is an antioxidant, preventing damage to important cellular components caused by reactive oxygen species such as free radicals and peroxides. Thiol groups are reducing agents, existing at a concentration of approximately 5 mM in animal cells. Glutathione reduces disulfide bonds formed within cytoplasmic proteins to cysteines by serving as an electron donor. In the process, glutathione is converted to its oxidized form, glutathione disulfide (GSSG), also called L-(–)-glutathione. Once oxidized, glutathione can be reduced back by glutathione reductase, using NADPH as an electron donor. The ratio of reduced glutathione to oxidized glutathione within cells is often used as a measure of cellular toxicity.
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