GluN1-NR1 Antibody : HRP (OASE00258-HRP)

Catalog No: OASE00258-HRP

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Monoclonal
• Clone: S308-48
• Isotype: IgG1
• Host: Mouse
• Conjugation: HRP: Horseradish Peroxidase
• Application: WB, IHC, ICC
• Additional Information: Background Info: Detects ~105kDa.
  Scientific Background: The NMDA receptor (NMDAR), a glutamate receptor, is the predominant molecular device for controlling synaptic plasticity and memory function (1). The NMDA receptor forms a heterotetramer between two NR1 and two NR2 subunits (the subunits are also called glutamate-binding NMDA receptor subunits or GluN for short); two obligatory NR1 subunits and two regionally localized NR2 subunits. A related gene family of NR3 A and B subunits have an inhibitory effect on receptor activity. Multiple receptor isoforms with distinct brain distributions and functional properties arise by selective splicing of the NR1 transcripts and differential expression of the NR2 subunits.
  Certificate of Analysis: 1ug/mL was sufficient for detection of NR1 glutamate receptor in 20ug of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Fusion protein amino acids 42-361 (extracellular N-terminus) of rat NR1
• Purification: Protein G Purified
• Concentration: 0.6-0.7 mg/ml
• Dilution: 1ug/mL (WB), 0.1-1ug/mL (IHC/ICC), 1-10ug/mL (IF)
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Li F., and Tsien J.Z. (2009) Clinical Implications of Basic Research: Memory and the NMDA receptors. New England J. Medicine. 361: 302-303.
  2. Garcia-Gallo M., Renart J., Diaz-Guerra M. (2001) Biochem J. 356: 539-547.
  3. Atlason P.T., Garside M.L., Meddows E., Whiting P., McIllhinney R.A.J. (2007) J Biol Chem. 282(35): 25299-25307.
  

Target Info

• Gene Symbol: Grin1
• Gene Full Name: glutamate receptor, ionotropic, N-methyl D-aspartate 1
• Alias Symbols: NR1, GluN1, NMDAR1
• NCBI Gene Id: 24408
• Uniprot ID: P35439
• Protein Accession #: NP_058706.1