GLUL ELISA Kit (Rat) (OKCD08530)

Catalog No: OKCD08530

Size:96WELLS

GLUL ELISA Kit (Rat) (OKCD08530) Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamine synthetase (GS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamine synthetase (GS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamine synthetase (GS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glutamine synthetase (GS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.62-1000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamine synthetase (GS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamine synthetase (GS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • GLUL Microplate: 96 Wells (12 x 8 Well strips)
  • GLUL Lyophilized Standard: 2
  • 100X GLUL HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 6.2pg/mL

Target Info

• Gene Symbol: Glul
• Gene Full Name: glutamate-ammonia ligase
• Alias Symbols: Glns;glutamate decarboxylase;Glutamate--ammonia ligase;glutamate-ammonia ligase (glutamine synthase);glutamate-ammonia ligase (glutamine synthetase);glutamine synthetase;Glutamine synthetase (glutamate-ammonia ligase);glutamine synthetase 1;GS;palmitoyltransferase GLUL.
• NCBI Gene Id: 24957
• Protein Name: Glutamine synthetase
• Description of Target: Essential for proliferation of fetal skin fibroblasts. This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity).
• Uniprot ID: P09606
• Protein Accession #: NP_058769.4
• Nucleotide Accession #: NM_017073.3