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Catalog No: OKEH00257
Size:96T
Price: $525.00
SKU
OKEH00257
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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GLUL ELISA Kit (Human) : 96 Wells (OKEH00257)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology GLUL ELISA Kit (Human) (OKEH00257) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GLUL has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for GLUL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GLUL captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.7% (n = 20)
Mean Inter-assay CV%: < 8.3% (n = 20)
ELISA Kit Component
ComponentAmount
GLUL Microplate96 Wells (12 x 8 Well strips)
GLUL Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated GLUL Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity9.1 pg/mL
SpecificityNatural and recombinant Human Glutamine synthetase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGLUL
Gene Full Nameglutamate-ammonia ligase
Alias Symbolscell proliferation-inducing protein 59;GLNS;glutamate decarboxylase;Glutamate--ammonia ligase;glutamine synthase;glutamine synthetase;GS;palmitoyltransferase GLUL;PIG43;PIG59;proliferation-inducing protein 43.
NCBI Gene Id2752
Protein NameP15104
Description of TargetGlutamine synthetase
Uniprot IDhttps://www.uniprot.org/uniprot/P15104
Protein Accession #NP_001028216.1
Nucleotide Accession #NM_001033044.3
Protein Size (# AA)373
Molecular Weight42 kDa
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