GLUL Chemi-Luminescent ELISA Kit (Mouse) (OKCD03545)

Catalog No: OKCD03545

Size:96WELLS

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	95-102%	90-102%	86-96%	87-101%
  EDTA Plasma (n=5)	88-102%	78-95%	88-103%	97-104%
  Heparin Plasma (n=5)	92-105%	82-93%	99-105%	82-96%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Glutamine synthetase (GS). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Glutamine synthetase (GS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Glutamine synthetase (GS) level in the sample or standard.
• ELISA Kit Range: 0.27-200pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	89-96	93
  EDTA Plasma (n=5)	83-97	92
  Heparin Plasma (n=5)	85-99	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamine synthetase (GS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamine synthetase (GS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Glul Microplate: 96 Wells (12 x 8 Well strips)
  • Glul Lyophilized Standard: 2
  • 100X Glul Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sensitivity: 0.27 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Glul
• Gene Full Name: glutamate-ammonia ligase (glutamine synthetase)
• Alias Symbols: G;Glns;glutamate decarboxylase;Glutamate--ammonia ligase;glutamate-ammonia ligase (glutamine synthase);glutamine synthetase;GS;palmitoyltransferase GLUL.
• NCBI Gene Id: 14645
• Protein Name: Glutamine synthetase
• Description of Target: Essential for proliferation of fetal skin fibroblasts. This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity).
• Uniprot ID: P15105
• Protein Accession #: NP_032157
• Nucleotide Accession #: NM_008131