GLUL Antibody - N-terminal region (OAAB04005)

Catalog No: OAAB04005

Size:400ul

Western blot analysis of GLUL Antibody (N-term) in mouse cerebellum tissue lysates (35ug/lane). GLUL (arrow) was detected using the purified Pab.

Western blot analysis of lysates from Hela, HT-29, Jurkat cell line, mouse brain, mouse cerebellum, rat cerebellum tissue lysate(from left to right), using GLUL Antibody (N-term)(Cat. #OAAB04005). OAAB04005 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

Formalin-fixed and paraffin-embedded human testis tissue reacted with GLUL Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

OAAB04005 staining GLUL in Human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: IHC-P, WB
• Additional Information: Function: This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner. Essential for proliferation of fetal skin fibroblasts.
  Cellular Location: Cytoplasm. Mitochondrion
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This GLUL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-96 amino acids from the N-terminal region of human GLUL.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Bovine, Chicken, Monkey, Pig
• Concentration: 0.5 mg/ml
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: IHC-P: 1:25
  WB: 1:2000

Target Info

• Gene Symbol: GLUL
• Alias Symbols: GS, GLNS, PIG43, PIG59
• NCBI Gene Id: 2752
• Protein Name: Glutamine synthetase
• Description of Target: GLUL belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling.
• Uniprot ID: P15104, P46410, P15105, Q4R7U3, P16580, P15103
• Protein Accession #: NP_001028216.1
• Molecular Weight: 42 kDa