GLUD1 Antibody (OAEB01763)

Catalog No: OAEB01763 (Formerly GWB-EV0806)

Size:100UG

OAEB01763 (0.3ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB01763 (3.75 ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: P-WB, IHC, IF
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: GLUD1 / GLUD2
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow; Dog; Human; Mouse; Rat
• Peptide Sequence: C-ESEEQKRNRVRGILR
• Concentration: 0.5 mg/ml
• Specificity: This antibody is expected to recognize both reported isoforms (NP_005262.1)
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:32000.
  Western blot: Approx 55kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates and in Human, Mouse and Rat Liver lysates (calculated MW of 61.4kDa according to Human NP_005262.1, of 61.3kDa according to Mouse NP_032159.1 and of 61.4kDa according to Rat NP_036702.1). Recommended concentration:0.3-1μg/ml.Primary incubation was 1 hour.
  IHC: In paraffin embedded Human Cerebral Cortex shows mitochondrial staining in neuronal cell bodies and axons. Recommended concentration: 3-5 μg/ml.
  Immunofluorescence: Found particulate cytoplasm staining in HeLa.
  Optimal dilutions should be determined by the end user.
• Reference: Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
  A high-throughput pipeline for validation of antibodies
  Nat Methods. 2018 Nov;15(11):909-912
  PMID: 30377371

Target Info

• Gene Symbol: Glud1|GLUD1|GLUD2
• Gene Full Name: glutamate dehydrogenase 1|glutamate dehydrogenase 2
• Alias Symbols: Ac2-281;AI118167;epididymis secretory sperm binding protein;epididymis tissue sperm binding protein Li 18mP;GDH;GDH 1;GDH 2;GDH1;GDH2;Gdh-X;Glu;Glud;Gludeha;Gludl;GLUDP1;glutamate dehydrogenase (NAD(P)+);glutamate dehydrogenase 1, mitochondrial;glutamate dehydrogenase 2, mitochondrial;glutamate dehydrogenase pseudogene 1;memory-related gene 2 protein;MRG-2;testicular secretory protein Li 14.
• NCBI Gene Id: 14661|24399|2746|2747
• Protein Name: Glutamate dehydrogenase 1, mitochondrial
• Description of Target: Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
• Uniprot ID: P00367
• Protein Accession #: NP_005262.1
• Molecular Weight: 61.4