Catalog No: OKEI00272
Size:96 Wells
Price: $625.00
SKU
OKEI00272
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- Phone: 858-552-6979
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Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Human | ||||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology GLTP ELISA Kit (Human) (OKEI00272) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GLTP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GLTP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample GLTP | ||||||||||||||||||||||
ELISA Kit Range | 0.016 - 1 ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level GLTP. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level GLTP using 8 replicates on each of 3 plates. Intra-Assay CV <10% Inter-Assay CV <12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Biological Fluids | ||||||||||||||||||||||
Sensitivity | 9.375 pg/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | GLTP |
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Alias Symbols | Glycolipid transfer protein |
NCBI Gene Id | 51228 |
Protein Name | Glycolipid transfer protein |
Description of Target | Accelerates the intermembrane transfer of various glycolipids. Catalyzes the transfer of various glycosphingolipids between membranes but does not catalyze the transfer of phospholipids. May be involved in the intracellular translocation of glucosylceramides. |
Uniprot ID | Q9NZD2 |
Protein Accession # | NP_057517 |
Nucleotide Accession # | NM_016433 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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