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Datasheets/Manuals | Printable datasheet for GLS2 Antibody (OAPB01260) (OAPB01260) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid. GLS2 Antibody is supplied in PBS containing 0.02% sodium azide. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | Unconjugated |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot |
Additional Information | Rat Liver Lysate (OCPB00063) |
:: | Buffer: Antibody is supplied in PBS containing 0.02% sodium azide. |
Reconstitution and Storage | 2°C to 8°C |
Immunogen | GLS2 antibody was raised against an 18 amino acid synthetic peptide near the center terminus of human GLS2. The immunogen is located within amino acids 300 - 350 of GLS2. |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Rat |
Concentration | 1 mg/ml |
Specificity | GLS2 antibody is predicted to not cross-react with other catenin family members. Multiple isoforms of GLS2 are known to exist. |
Application Info | GLS2 antibody can be used for detection of GLS2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
Reference | Chung-Bok MI, Vincent N, Jhala U, et al. Rat hepatic glutaminase: identification of the full coding sequence and characterization of a functional promoter. Biochem. J. 1997; 324:193-200.;Gomez-Fabre PM, Aledo JC, del Castillo-Olivares A, et al. Molecular cloning, sequencing and expression studies of the human breast cancer cell glutaminase. Biochem. J. 2000; 345:365-75. ;Hu W, Zhang C, Wu R, et al. Glutaminase 2, a novel p53 target gene regulating energy metabolism and antioxidant function. Proc. Natl. Acad. Sci. USA 2010; 107:7455-60.; |
Gene Symbol | GLS2 |
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Gene Full Name | glutaminase 2 |
Alias Symbols | breast cell glutaminase;GA;GLS;glutaminase 2 (liver, mitochondrial);glutaminase I;glutaminase liver isoform, mitochondrial;hLGA;LGA;L-glutaminase;L-glutamine amidohydrolase;phosphate-activated glutaminase;phosphate-dependent glutaminase. |
NCBI Gene Id | 27165 |
Protein Name | Glutaminase liver isoform, mitochondrial|glutaminase liver isoform, mitochondrial isoform 1 precursor [Homo sapiens] |
Description of Target | Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation. |
Uniprot ID | Q9UI32 |
Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_037399 |
Molecular Weight | Predicted: 36, 55, 62, 66 kDa Observed: 55, 62 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "GLS2 Antibody (OAPB01260)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "GLS2 Antibody (OAPB01260)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "GLS2 Antibody (OAPB01260)" provided in?
This item is provided in "Liquid. GLS2 Antibody is supplied in PBS containing 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "GLS2 Antibody (OAPB01260)"?
This target may also be called "breast cell glutaminase;GA;GLS;glutaminase 2 (liver, mitochondrial);glutaminase I;glutaminase liver isoform, mitochondrial;hLGA;LGA;L-glutaminase;L-glutamine amidohydrolase;phosphate-activated glutaminase;phosphate-dependent glutaminase." in publications.
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What is the shipping cost for "GLS2 Antibody (OAPB01260)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "GLS2 Antibody (OAPB01260)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "GLS2 Antibody (OAPB01260)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "Predicted: 36, 55, 62, 66 kDa Observed: 55, 62 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "GLS2 Antibody (OAPB01260)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "GLS2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "GLS2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "GLS2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "GLS2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "GLS2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "GLS2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.