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GLS2 Antibody - C-terminal region (OAAB03686)

  • Catalog#: OAAB03686
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Predicted Species Reactivity:
Human
Product Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Host:
Rabbit
Application:
WB, IHC, FC
Additional Information:
Function:Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
:::
Cellular Location: Mitochondrion.
Tissue Location: Highly expressed in liver. Expressed in brain and pancreas. Not observed in heart, placenta, lung, skeletal muscle and kidney. Expression is significantly reduced in hepatocellular carcinomas
Reconstitution and Storage:
Maintain refrigerated at 2-8C for up to 6 months. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Gene Symbol:
GLS2
NCBI Gene Id:
27165
Protein Name:
Glutaminase liver isoform, mitochondrial
Swissprot Id:
Q9UI32
Protein Accession #:
NP_037399.2
Alias Symbols:
Glutaminase liver isoform, mitochondrial, GLS, L-glutamine amidohydrolase, L-glutaminase, GA, GLS2
Replacement Item:
This antibody may replace item sc-168424 from Santa Cruz Biotechnology.
Molecular Weight:
66323 Da
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express GLS2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express GLS2.
Predicted Homology Based on Immunogen Sequence:
Human
Datasheets/Manuals:
Printable datasheet for anti-GLS2 antibody - OAAB03686
Specificity:
This GLS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 463~493 amino acids from the C-terminal region of human GLS2.
Application Info:
The suggested dilutions are:
Western Blot ~~ 1:1,000
Immunohistochemistry ~~ 1:10~50
Flow cytometry~~1:10~50
Other Applications Image 1 Data:
Flow cytometric analysis of K562 cells using GLS2 Antibody (C-term R471)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Publications:

Shimmura, C. et al. Enzymes in the glutamate-glutamine cycle in the anterior cingulate cortex in postmortem brain of subjects with autism. Mol. Autism 4, 6 (2013). WB, IHC, FC, Human 23531457

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