- Predicted Species Reactivity:
- Human
- Product Format:
- Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Clonality:
- Polyclonal
- Isotype:
- Rabbit Ig
- Host:
- Rabbit
- Application:
- WB, IHC, FC
- Additional Information:
- Function:Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
- :::
- Cellular Location: Mitochondrion.
Tissue Location: Highly expressed in liver. Expressed in brain and pancreas. Not observed in heart, placenta, lung, skeletal muscle and kidney. Expression is significantly reduced in hepatocellular carcinomas - Reconstitution and Storage:
- Maintain refrigerated at 2-8C for up to 6 months. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
- Gene Symbol:
- GLS2
- NCBI Gene Id:
- 27165
- Protein Name:
- Glutaminase liver isoform, mitochondrial
- Swissprot Id:
- Q9UI32
- Protein Accession #:
- NP_037399.2
- Alias Symbols:
- Glutaminase liver isoform, mitochondrial, GLS, L-glutamine amidohydrolase, L-glutaminase, GA, GLS2
- Replacement Item:
- This antibody may replace item sc-168424 from Santa Cruz Biotechnology.
- Molecular Weight:
- 66323 Da
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express GLS2.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express GLS2.
- Predicted Homology Based on Immunogen Sequence:
- Human
- Datasheets/Manuals:
- Printable datasheet for anti-GLS2 antibody - OAAB03686
- Specificity:
- This GLS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 463~493 amino acids from the C-terminal region of human GLS2.
- Application Info:
- The suggested dilutions are:
Western Blot ~~ 1:1,000
Immunohistochemistry ~~ 1:10~50
Flow cytometry~~1:10~50 - Other Applications Image 1 Data:
- Flow cytometric analysis of K562 cells using GLS2 Antibody (C-term R471)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Publications:
Shimmura, C. et al. Enzymes in the glutamate-glutamine cycle in the anterior cingulate cortex in postmortem brain of subjects with autism. Mol. Autism 4, 6 (2013). WB, IHC, FC, Human 23531457
Product Reviews
