GLS2 Antibody - C-terminal region (OAAB03686)

Catalog#: OAAB03686

• Size: 400ul

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC, FC
• Additional Information: Function:Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
  Cellular Location: Mitochondrion.
  Tissue Location: Highly expressed in liver. Expressed in brain and pancreas. Not observed in heart, placenta, lung, skeletal muscle and kidney. Expression is significantly reduced in hepatocellular carcinomas
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 6 months. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Gene Symbol: GLS2
• NCBI Gene Id: 27165
• Protein Name: Glutaminase liver isoform, mitochondrial
• Swissprot Id: Q9UI32
• Protein Accession #: NP_037399.2
• Alias Symbols: Glutaminase liver isoform, mitochondrial, GLS, L-glutamine amidohydrolase, L-glutaminase, GA, GLS2
• Replacement Item: This antibody may replace item sc-168424 from Santa Cruz Biotechnology.
• Molecular Weight: 66323 Da
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express GLS2.
• Predicted Homology Based on Immunogen Sequence: Human
• Specificity: This GLS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 463~493 amino acids from the C-terminal region of human GLS2.
• Application Info: The suggested dilutions are:
  Western Blot ~~ 1:1,000
  Immunohistochemistry ~~ 1:10~50
  Flow cytometry~~1:10~50
• Other Applications Image 1 Data: Flow cytometric analysis of K562 cells using GLS2 Antibody (C-term R471)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Publications:

  Shimmura, C. et al. Enzymes in the glutamate-glutamine cycle in the anterior cingulate cortex in postmortem brain of subjects with autism. Mol. Autism 4, 6 (2013). WB, IHC, FC, Human 23531457

Protocol:

Tips Information:

Product Protocol: GLS2 antibody tested with human K562 by western blot (OAAB03686)

Product Protocol: GLS2 antibody tested with human brain in immunohistochemistry (IHC) (OAAB03686)