Catalog No: OKEH02281
Size:96T
Price: $625.00
SKU
OKEH02281
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology GLRX ELISA Kit (Mouse) (OKEH02281) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GLRX has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GLRX is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GLRX captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 84%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 7.7% (n = 20)
ELISA Kit Component
ComponentAmount
GLRX Microplate96 Wells (12 x 8 Well strips)
GLRX Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated GLRX Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.089 ng/mL
SpecificityNatural and recombinant Mouse Glutaredoxin-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGlrx
Gene Full Nameglutaredoxin
Alias SymbolsC86710;D13Wsu156;D13Wsu156e;Glrx1;glutaredoxin (thioltransferase);glutaredoxin 1 (thioltransferase);glutaredoxin-1;Grx;Grx1;thioltransferase-1;TTa;TTase;TTase-1.
NCBI Gene Id93692
Protein NameQ9QUH0
Description of TargetGlutaredoxin-1
Uniprot IDhttps://www.uniprot.org/uniprot/Q9QUH0
Protein Accession #NP_444338.2
Nucleotide Accession #NM_053108.4
Protein Size (# AA)107
Molecular Weight12 kDa
Write Your Own Review
You're reviewing:GLRX ELISA Kit (Mouse) (OKEH02281)
Your Rating
We found other products you might like!