Catalog No: OKCD02572
Size:96WELLS
Price: $675.00
SKU
OKCD02572
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Datasheets/ManualsPrintable datasheet for GLRX ELISA Kit (Human) (OKCD02572)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)96-105%78-99%79-93%93-101%
EDTA Plasma (n=5)90-104%94-105%79-101%87-101%
Heparin Plasma (n=5)78-97%87-101%80-91%89-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutaredoxin (GLRX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutaredoxin (GLRX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutaredoxin (GLRX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glutaredoxin (GLRX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.94-60ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)96-105102
EDTA Plasma (n=5)83-9691
Heparin Plasma(n=5)91-10197
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutaredoxin (GLRX) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutaredoxin (GLRX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-GLRX Microplate96 Wells (12 x 8 Well strips)
GLRX Lyophilized Standard2
100X Biotinylated GLRX Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity28.9 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Glutaredoxin (GLRX). No significant cross-reactivity or interference between Glutaredoxin (GLRX) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolGLRX
Gene Full Nameglutaredoxin
Alias Symbolsglutaredoxin-1;GRX;GRX1;thioltransferase;thioltransferase-1;TTase-1.
NCBI Gene Id2745
Protein NameGlutaredoxin-1
Description of TargetHas a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.
Uniprot IDP35754
Protein Accession #NP_001112362.1
Nucleotide Accession #NM_001118890.1
Protein Size (# AA)106
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