GLRA2 ELISA Kit (Rat) (OKCD00952)

Catalog No: OKCD00952 (Formerly GWB-KBBMC6)

Size:96WELLS

Rat Glra2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  	81-98%	83-90%	89-104%	90-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycine Receptor Alpha 2 (GLRa2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycine Receptor Alpha 2 (GLRa2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycine Receptor Alpha 2 (GLRa2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glycine Receptor Alpha 2 (GLRa2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  	93-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycine Receptor Alpha 2 (GLRa2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycine Receptor Alpha 2 (GLRa2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Glra2 Microplate: 96 Wells (12 x 8 Well Strips)
  • Glra2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated Glra2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.115 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glycine Receptor Alpha 2 (GLRa2). No significant cross-reactivity or interference between Glycine Receptor Alpha 2 (GLRa2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Glra2
• Gene Full Name: glycine receptor, alpha 2
• Alias Symbols: Glycine receptor alpha 2 subunit (glycine receptor, neonatal);glycine receptor strychnine-binding subunit;glycine receptor subunit alpha-2;glycine receptor subunit alpha-2-like;RNNEOGLY.
• NCBI Gene Id: 24397
• Protein Name: Glycine receptor subunit alpha-2
• Description of Target: Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine. Channel opening is also triggered by taurine and beta-alanine (PubMed:2176511). Plays a role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents. Plays a role in cellular responses to ethanol.
• Uniprot ID: P22771
• Protein Accession #: NP_036700
• Nucleotide Accession #: NM_012568.3
• Protein Size (# AA): 452