Size:200 ug
$309.00
In Stock
SKU
OAPC00117
Request Bulk
Order Quote
Aviva's
Satisfaction
Guarantee
Contact Us:
  • Toll Free: (888)880-0001
  • Phone: (858)552-6979
  • Email: info@avivasysbio.com
Shipping Info:
  • $40: Antibody & Protein in US
  • $50: 1-2 Kits in US
  • Contact us for international orders.

GLRA1 Antibody (OAPC00117)

Catalog#: OAPC00117
Domestic: within 1 week delivery | International: 1 week
This product is Genway GWB-C33F7E
More Information
Predicted Species Reactivity Human, Mouse, Rat
Product Format Lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.
Clonality Polyclonal
Host Rabbit
Application WB, IHC, DB
Reconstitution and Storage Packaging: 200 ug antibody vial; lyophilized from 5 mM ammonium bicarbonate.Adequate amount of material to conduct 10-mini Western Blots.
Reconstitution: The antibody should be reconstituted in 100 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2HPO4, pH 7.4) before use.
Storage: After reconstitution the antibody should be aliquoted and stored at -20C.
Replacement Item This antibody may replace item sc-133629 from Santa Cruz Biotechnology.
Immunogen Peptide from the N-terminus region of the alpha 1-subunit of rat glycine receptor.
Purification Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.
Predicted Homology Based on Immunogen Sequence Human, Mouse, Rat
Datasheets/Manuals Printable datasheet for OAPC00117
Specificity Specific for the ~48k alpha 1- and alpha 2-subunits of the glycine receptor in Western blots of rat spinal cord and brain stem and in cell extracts containing the recombinant human Glycine Receptors. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen derived from the amino terminus of the alpha1 subunit. Does not recognize other glycine receptor subunits.
Application Info The antibody has been directly tested for reactivity in Western blots in human, mouse and rat tissues.
Western blot of rat spinal cord showing specific immunolabeling of the ~48k alpha 1- and alpha 2-subunits of the glycine receptor. The labeling was absent from a rat hippocampal (hipp) lysate as the glycine receptor is not expressed in the hippocampus.
Working Dilutions:
DB~~1:1000
IHC~~1:1000
WB~~ 1:1000
Target Reference Beckstead MJ, Phelan R, Mihic SJ (2001) Antagonism of inhalant and volatile anesthetic enhancement of glycine receptor function. J Biol Chem 276:24959-24964.
Chatterton JE, Awobuluyi M, Premkumar LS, Takahashi H, Talantova M, Shin Y, Cui JK, Tu SC, Kevin ASK, Nakanishi N, Tong G, Lipton SA, Zhang DX (2002) Excitatory glycine receptors containing the NR3 family of NMDA receptor subunits. Nature (London) 415:793-798.
Craig AM, Lichtman JW (2001) Getting a bead on receptor movements. Nat Neurosci 4:219-220.
Fischer F, Kneussel M, Tintrup H, Haverkamp S, Rauen T, Betz H, Wässle H (2000) Reduced synaptic clustering of GABA and glycine receptors in the retina of the gephyrin null mutant mouse. J Comp Neurol 427:634-648.
Mihic SJ, Ye Q, Wick MJ, Koltchine VV, Krasowski MD, Finn SE, Mascia MP, Valenzuela CF, Hanson KK, Greenblatt EP, Harris RA, Harrison NL (1997) Sites of alcohol and volatile anaesthetic action on GABAA and glycine receptors. Nature (London) 389:385-389.
Schofield CM, Jenkins A, Harrison NL (2003) A highly conserved aspartic acid residue in the signature disulfide loop of the alpha 1-subunit is a determinant of gating in the glycine receptor. J Biol Chem 278:34079-34083.
Publications

Zeng, J; Ekman, M; Jiang, C; Uvelius, B; Swärd, K; Non-uniform changes in membrane receptors in the rat urinary bladder following outlet obstruction. 762, 82-8 (2015). WB, IHC, DB, Human, Mouse, Rat 26004535

Target GLRA1, GLRA2
Alias Symbols GLYR, GLRA1, GLRA2
NCBI Gene Id 25674; 24397
Protein Name Glycine receptor subunit alpha-1, Glycine receptor subunit alpha-2
Description of Target Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003). The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000). The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins (Beckstead et al., 2001; Mihic et al., 1997). These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001). The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002).
Swissprot Id P07727, P22771
Protein Accession # NP_037265.1, NP_036700.1
Molecular Weight ~48kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express GLRA1.
RNA Seq Find tissues and cell lines supported by DNA array analysis to express GLRA1.
  1. What is the species homology for "GLRA1 Antibody (OAPC00117)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".

  2. How long will it take to receive "GLRA1 Antibody (OAPC00117)"?

    This item is available "Domestic: within 1 week delivery | International: 1 week".

  3. What buffer format is "GLRA1 Antibody (OAPC00117)" provided in?

    This item is provided in "Lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "GLRA1 Antibody (OAPC00117)"?

    This target may also be called "GLYR, GLRA1, GLRA2" in publications.

  5. What is the shipping cost for "GLRA1 Antibody (OAPC00117)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "GLRA1 Antibody (OAPC00117)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "GLRA1 Antibody (OAPC00117)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "~48kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "GLRA1 Antibody (OAPC00117)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for "GLRA1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "GLRA1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "GLRA1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "GLRA1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "GLRA1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "GLRA1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

Write Your Own Review
You're reviewing:GLRA1 Antibody (OAPC00117)
Your Rating