GLI3 Antibody (OAGA02285)

Catalog No: OAGA02285

Size:100 ul

GLI3 antibody detects GLI3 protein by Western blot analysis.
A. 30 ug PC-12 whole cell lysate/extract
B. 30 ug Rat2 whole cell lysate/extract
5 % SDS-PAGE
GLI3 antibody (OAGA02285) dilution: 1:1000

Sample (20 ug of whole cell lysate)
A: mouse ESC
5% SDS PAGE
OAGA02285 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: Jurkat
5% SDS PAGE
OAGA02285 diluted at 1:1000

GLI3 antibody detects GLI3 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
GLI3 antibody (OAGA02285) diluted at 1:250.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GLI3 (OAGA02285) antibody at 1:500 dilution.

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid 1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, ICC, IF, IHC-P
• Additional Information: Positive Control: Jurkat, mouse ESC, PC-12, Rat2
  Cellular Localization: Nucleus
• Reconstitution and Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze/thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GLI3. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Predicted Homology Based on Immunogen Sequence: Chicken, Xenopus laevis, Chimpanzee (>80% identity)
• Concentration: 1 mg/ml
• Application Info: WB: 1:500~3000
  ICC/IF: 1:100~1000
  IHC-P: 1:100~1000

Target Info

• Gene Symbol: GLI3
• Gene Full Name: GLI family zinc finger 3
• Alias Symbols: PHS, ACLS, GCPS, PAPA, PAPB, PAP-A, PAPA1, PPDIV, GLI3FL, GLI3-190
• NCBI Gene Id: 2737
• Description of Target: This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B.
• Uniprot ID: P10071
• Molecular Weight: 170 kDa