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GLI3 Antibody (OAEB00253)

100 ug
$229.00
In Stock
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Gene Symbol:
GLI3
NCBI Gene Id:
2737
Protein Name:
Transcriptional activator GLI3
Swissprot Id:
P10071
Protein Accession #:
NP_000159.2
Alias Symbols:
GLI3, GLI-Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome), ACLS, GCPS, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV, DNA-binding protein, GLI-Kruppel family member GLI3, oncogene GLI3, zinc finger protein GLI3
Replacement Item:
This antibody may replace item sc-20688 from Santa Cruz Biotechnology.
Host:
Goat
Clonality:
Polyclonal
Application:
ELISA
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express GLI3.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express GLI3.
Predicted Species Reactivity:
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Complete computational species homology data:
GLI3 Antibody (OAEB00253)
Peptide Sequence:
C-HNKRSKIKPDED
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals:
Printable datasheet for anti-GLI3 antibody - OAEB00253
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:16000.
Western Blot: Preliminary experiments in Human Brain, Lung, Placenta and Testes lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).
Target Reference:
* Haycraft CJ, Banizs B, Aydin-Son Y, Zhang Q, Michaud EJ, Yoder BK.; Gli2 and gli3 localize to cilia and require the intraflagellar transport protein polaris for processing and function.; PLoS Genet. 2005 Oct;1(4):e53. Epub 2005 Oct 28.; Pubmed 16254602

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