GLI2 Antibody (OARA02754)

Catalog No: OARA02754 (Formerly GWB-CE7858)

Size:100UG

Western blot using Aviva's affinity purified anti-Gli-2 antibody shows detection of Gli-2 protein in rat testes (lane 1) and human HEK293 (lane 2) whole cell lysates (arrowhead). See Ruppert et al for testing conditions. Each lane contains approximately 35 ug of lysate. Primary antibody was used at a 1:400 dilution in 5% BLOTTO in PBS overnight at 4C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Affinity Purified anti-Gli2 antibody shows strong cytoplasmic and membranous staining of tumor cells in human breast tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.

Product Info

• Predicted Species Reactivity: Human|Rat
• Product Format: Liquid (sterile filtered)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-65 of human Gli-2 (isoform a).
• Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.
• Concentration: 1 mg/ml
• Specificity: This affinity-purified antibody is directed against human Gli-2 protein. A BLAST analysis was used to suggest cross reactivity with Gli-2 from human and chimpanzee based on the immunizing sequence.
• Application Info: ELISA: 1:10000~30000
  IHC: 1:500~2000
  WB: 1:500~2000
  This antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. See figure legend for expectations by western blot. Multiple splice variants have been reported for this protein a, b, g and d (133.3, 131.6, 88.1 and 86.4 kDa respectively). Detection of Gli-2 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-2 is likely to be low. Furthermore, Gli-2 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.
• Reference: Roessler,E., Ermilov,A.N., Grange,D.K., Wang,A., Grachtchouk,M., Dlugosz,A.A. and Muenke,M. (2005) A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2. Hum. Mol. Genet. 14 (15), 2181-2188.
  Ikram,M.S., Neill,G.W., Regl,G., Eichberger,T., Frischauf,A.M., Aberger,F., Quinn,A. and Philpott,M. (2004) GLI2 is expressed in normal human epidermis and BCC and induces GLI1 expression by binding to its promoter. J. Invest. Dermatol. 122 (6), 1503-1509.
  Regl,G., Kasper,M., Schnidar,H., Eichberger,T., Neill,G.W., Ikram,M.S., Quinn,A.G., Philpott,M.P., Frischauf,A.M. and Aberger,F. (2004) The zinc-finger transcription factor GLI2 antagonizes contact inhibition and differentiation of human epidermal cells. Oncogene 23 (6), 1263-1274.
• Publications:

  Anti-Gli-2 OARA02754 has recently been referenced in the following publications:

  Schneider, S. et al. Expression of the Sonic hedgehog pathway in squamous cell carcinoma of the skin and the mucosa of the head and neck. Head Neck 33, 244-50 (2011). 20848446

Target Info

• Gene Symbol: GLI2
• Gene Full Name: GLI family zinc finger 2
• Alias Symbols: CJS;GLI family zinc finger protein 2;GLI-Kruppel family member GLI2;glioma-associated oncogene family zinc finger 2;HPE9;oncogene GLI2;PHS2;Tax helper protein;tax helper protein 1;tax helper protein 2;tax-responsive element-2 holding protein;tax-responsive element-25-bp sequence binding protein;THP1;THP2;zinc finger protein GLI2.
• NCBI Gene Id: 2736
• Protein Name: Zinc finger protein GLI2|zinc finger protein GLI2 [Homo sapiens]
• Description of Target: Functions as transcription regulator in the hedgehog (Hh) pathway (PubMed:18455992, PubMed:26565916). Functions as transcriptional activator (PubMed:9557682, PubMed:19878745, PubMed:24311597). May also function as transcriptional repressor (By similarity). Requires STK36 for full transcriptional activator activity. Required for normal embryonic development (PubMed:15994174, PubMed:20685856).
• Uniprot ID: P10070
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_005261.2