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Datasheets/Manuals | Printable datasheet for anti-GLI2 (ARP31885_T100-HRP) antibody |
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Predicted Species Reactivity | Human |
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Product Format | Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | HRP: Horseradish Peroxidase |
Application | IHC, WB |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human GLI2 |
Predicted Homology Based on Immunogen Sequence | Human: 100% |
Peptide Sequence | Synthetic peptide located within the following region: RNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTE |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-GLI2 (ARP31885_T100-HRP) antibody is Catalog # AAP31885 (Previous Catalog # AAPP02680) |
Reference | Ikram,M.S., et al., (2004) J. Invest. Dermatol. 122 (6), 1503-1509 |
Publications | Kiprilov, E. N. et al. Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery. J. Cell Biol. 180, 897-904 (2008). ICC/IF, Human 18332216 Schneider, S. et al. Expression of the Sonic hedgehog pathway in squamous cell carcinoma of the skin and the mucosa of the head and neck. Head Neck 33, 244-50 (2011). WB, Human 20848446 Tostar, U., Finta, C., Rahman, M. F.-U., Shimokawa, T. & Zaphiropoulos, P. G. Novel mechanism of action on Hedgehog signaling by a suppressor of fused carboxy terminal variant. PLoS One 7, e37761 (2012). WB, Human 22666390 Kim, Y. et al. Selective down-regulation of glioma-associated oncogene 2 inhibits the proliferation of hepatocellular carcinoma cells. Cancer Res. 67, 3583-93 (2007). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 17440069 Kabasawa, Y. et al. Amelogenin positive cells scattered in the interstitial component of odontogenic fibromas. J. Clin. Pathol. 61, 851-5 (2008). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 18344235 Omenetti, A. et al. The hedgehog pathway regulates remodelling responses to biliary obstruction in rats. Gut 57, 1275-82 (2008). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 18375471 Omenetti, A. et al. Hedgehog signaling regulates epithelial-mesenchymal transition during biliary fibrosis in rodents and humans. J. Clin. Invest. 118, 3331-42 (2008). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 18802480 Takanaga, H. et al. Gli2 is a novel regulator of sox2 expression in telencephalic neuroepithelial cells. Stem Cells 27, 165-74 (2009). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 18927476 Madison, B. B., McKenna, L. B., Dolson, D., Epstein, D. J. & Kaestner, K. H. FoxF1 and FoxL1 link hedgehog signaling and the control of epithelial proliferation in the developing stomach and intestine. J. Biol. Chem. 284, 5936-44 (2009). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 19049965 Wall, D. S. et al. Progenitor cell proliferation in the retina is dependent on Notch-independent Sonic hedgehog/Hes1 activity. J. Cell Biol. 184, 101-12 (2009). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 19124651 Syn, W.-K. et al. Role for hedgehog pathway in regulating growth and function of invariant NKT cells. Eur. J. Immunol. 39, 1879-92 (2009). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 19544307 Kim, J. E. et al. Sonic hedgehog signaling proteins and ATP-binding cassette G2 are aberrantly expressed in diffuse large B-cell lymphoma. Mod. Pathol. 22, 1312-20 (2009). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 19593328 Ho, L. et al. Gli2 and p53 cooperate to regulate IGFBP-3- mediated chondrocyte apoptosis in the progression from benign to malignant cartilage tumors. Cancer Cell 16, 126-36 (2009). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 19647223 Kim, W.-Y. et al. GSK-3 is a master regulator of neural progenitor homeostasis. Nat. Neurosci. 12, 1390-7 (2009). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 19801986 Nakamura, K. et al. Hedgehog promotes neovascularization in pancreatic cancers by regulating Ang-1 and IGF-1 expression in bone-marrow derived pro-angiogenic cells. PLoS One 5, e8824 (2010). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 20098680 Youssef, K. K. et al. Identification of the cell lineage at the origin of basal cell carcinoma. Nat. Cell Biol. 12, 299-305 (2010). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 20154679 Peacock, Z. S., Cox, D. & Schmidt, B. L. Involvement of PTCH1 mutations in the calcifying epithelial odontogenic tumor. Oral Oncol. 46, 387-92 (2010). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 20371205 Coon, V. et al. Molecular therapy targeting Sonic hedgehog and hepatocyte growth factor signaling in a mouse model of medulloblastoma. Mol. Cancer Ther. 9, 2627-36 (2010). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 20807782 Malek, R., Matta, J., Taylor, N., Perry, M. E. & Mendrysa, S. M. The p53 inhibitor MDM2 facilitates Sonic Hedgehog-mediated tumorigenesis and influences cerebellar foliation. PLoS One 6, e17884 (2011). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 21437245 Jung, I. H., Jung, D. E., Park, Y. N., Song, S. Y. & Park, S. W. Aberrant Hedgehog ligands induce progressive pancreatic fibrosis by paracrine activation of myofibroblasts and ductular cells in transgenic zebrafish. PLoS One 6, e27941 (2011). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 22164219 Yang, L. et al. Hedgehog signaling activation in the development of squamous cell carcinoma and adenocarcinoma of esophagus. Int. J. Biochem. Mol. Biol. 3, 46-57 (2012). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 22509480 Bosco-Clément, G. et al. Targeting Gli transcription activation by small molecule suppresses tumor growth. Oncogene 33, 2087-97 (2014). WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 23686308 Li, Y. et al. Gene expression profiling reveals the heterogeneous transcriptional activity of Oct3/4 and its possible interaction with Gli2 in mouse embryonic stem cells. Genomics 102, 456-67 WB, IHC, ICC/IF, IP, EMSA, ChIP, Human 24121003 |
Gene Symbol | GLI2 |
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Gene Full Name | GLI family zinc finger 2 |
Alias Symbols | CJS, HPE9, PHS2, THP1, THP2 |
NCBI Gene Id | 2736 |
Protein Name | Zinc finger protein GLI2 |
Description of Target | GLI2 encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. |
Uniprot ID | P10070 |
Protein Accession # | NP_084655 |
Nucleotide Accession # | NM_030379 |
Protein Size (# AA) | 1258 |
Molecular Weight | 133kDa |
Protein Interactions | SPOP; SKI; HDAC1; STK36; FBXW11; SUFU; ZIC2; ZIC1; CREB1; TBP; |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "GLI2 Antibody - middle region : HRP (ARP31885_T100-HRP)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "GLI2 Antibody - middle region : HRP (ARP31885_T100-HRP)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "GLI2 Antibody - middle region : HRP (ARP31885_T100-HRP)" provided in?
This item is provided in "Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "GLI2 Antibody - middle region : HRP (ARP31885_T100-HRP)"?
This target may also be called "CJS, HPE9, PHS2, THP1, THP2" in publications.
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What is the shipping cost for "GLI2 Antibody - middle region : HRP (ARP31885_T100-HRP)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "GLI2 Antibody - middle region : HRP (ARP31885_T100-HRP)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "GLI2 Antibody - middle region : HRP (ARP31885_T100-HRP)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "133kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "GLI2 Antibody - middle region : HRP (ARP31885_T100-HRP)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "GLI2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "GLI2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "GLI2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "GLI2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "GLI2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "GLI2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.