GLI1 ELISA Kit (Human) (OKCD02013)

Catalog No: OKCD02013 (Formerly GWB-KBBEX4)

Size:96WELLS

Human GLI1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: ELISA-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GLI Family Zinc Finger Protein 1 (GLI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GLI Family Zinc Finger Protein 1 (GLI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GLI Family Zinc Finger Protein 1 (GLI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GLI Family Zinc Finger Protein 1 (GLI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GLI Family Zinc Finger Protein 1 (GLI1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GLI Family Zinc Finger Protein 1 (GLI1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CILP2 Microplate: 96 Wells (12 x 8 Well Strips)
  • CILP2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated CILP2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 13 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.119 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of GLI Family Zinc Finger Protein 1 (GLI1). No significant cross-reactivity or interference between GLI Family Zinc Finger Protein 1 (GLI1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: GLI1
• Gene Full Name: GLI family zinc finger 1
• Alias Symbols: GLI;GLI-Kruppel family member GLI1;Glioma-associated oncogene;glioma-associated oncogene 1;glioma-associated oncogene homolog 1 (zinc finger protein);oncogene GLI;PAPA8;PPD1;zinc finger protein GLI1.
• NCBI Gene Id: 2735
• Protein Name: Zinc finger protein GLI1
• Description of Target: Acts as a transcriptional activator (PubMed:19706761, PubMed:10806483, PubMed:19878745, PubMed:24311597, PubMed:24217340). Binds to the DNA consensus sequence 5'-GACCACCCA-3' (PubMed:2105456, PubMed:8378770, PubMed:24217340). May regulate the transcription of specific genes during normal development (PubMed:19706761). May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling (PubMed:19706761). Plays a role in cell proliferation and differentiation via its role in SHH signaling (Probable).
• Uniprot ID: P08151
• Protein Accession #: NP_001153517.1
• Nucleotide Accession #: NM_001160045.1
• Protein Size (# AA): 1106