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Datasheets/Manuals | Printable datasheet for anti-GLI1 (ARP32368_T100-FITC) antibody |
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Predicted Species Reactivity | Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
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Product Format | Liquid. Purified antibody supplied in 1x PBS buffer. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | FITC: Fluorescein Isothiocyanate |
Application | WB |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | The immunogen is a synthetic peptide directed towards the C terminal region of human GLI1 |
Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100% |
Peptide Sequence | Synthetic peptide located within the following region: TNPSCGHPEVGRLGGGPALYPPPEGQVCNPLDSLDLDNTQLDFVAILDEP |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-GLI1 (ARP32368_T100-FITC) antibody is Catalog # AAP32368 (Previous Catalog # AAPP03357) |
Reference | Rahnama,F., et al., (2006) Biochem. J. 394 (PT 1), 19-26 |
Publications | Yoshimura, K., Kawate, T. & Takeda, S. Signaling through the primary cilium affects glial cell survival under a stressed environment. Glia 59, 333-44 (2011). WB, IP, Bovine, Human, Dog, Pig, Horse, Rabbit, Rat, Guinea pig, Mouse 21125655 Maitah, M. Y., Ali, S., Ahmad, A., Gadgeel, S. & Sarkar, F. H. Up-regulation of sonic hedgehog contributes to TGF-β1-induced epithelial to mesenchymal transition in NSCLC cells. PLoS One 6, e16068 (2011). WB, IP, Bovine, Human, Dog, Pig, Horse, Rabbit, Rat, Guinea pig, Mouse 21249152 Schiapparelli, P. et al. Inhibition of the sonic hedgehog pathway by cyplopamine reduces the CD133+/CD15+ cell compartment and the in vitro tumorigenic capability of neuroblastoma cells. Cancer Lett. 310, 222-31 (2011). WB, IP, Bovine, Human, Dog, Pig, Horse, Rabbit, Rat, Guinea pig, Mouse 21803487 Ferruzzi, P. et al. In vitro and in vivo characterization of a novel Hedgehog signaling antagonist in human glioblastoma cell lines. Int. J. Cancer 131, E33-44 (2012). WB, IP, Bovine, Human, Dog, Pig, Horse, Rabbit, Rat, Guinea pig, Mouse 22072503 Ortega, M. C. et al. Megalin mediates the influence of sonic hedgehog on oligodendrocyte precursor cell migration and proliferation during development. Glia 60, 851-66 (2012). WB, IP, Bovine, Human, Dog, Pig, Horse, Rabbit, Rat, Guinea pig, Mouse 22354480 Pandolfi, S. et al. WIP1 phosphatase modulates the Hedgehog signaling by enhancing GLI1 function. Oncogene 32, 4737-47 (2013). WB, IP, Bovine, Human, Dog, Pig, Horse, Rabbit, Rat, Guinea pig, Mouse 23146903 Bosco-Clément, G. et al. Targeting Gli transcription activation by small molecule suppresses tumor growth. Oncogene 33, 2087-97 (2014). WB, IP, Bovine, Human, Dog, Pig, Horse, Rabbit, Rat, Guinea pig, Mouse 23686308 Iwata, J. et al. Modulation of lipid metabolic defects rescues cleft palate in Tgfbr2 mutant mice. Hum. Mol. Genet. 23, 182-93 (2014). WB, IP, Bovine, Human, Dog, Pig, Horse, Rabbit, Rat, Guinea pig, Mouse 23975680 |
Gene Symbol | GLI1 |
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Gene Full Name | GLI family zinc finger 1 |
Alias Symbols | GLI, PPD1, PAPA8 |
NCBI Gene Id | 2735 |
Protein Name | Zinc finger protein GLI1 |
Description of Target | GLI1 is a protein which is a member of the Kruppel family of zinc finger proteins. The function of this gene has not been determined; however, it may play a role in normal development gene transcription. Mouse mutation studies indicate possible involvement in human foregut malformation. |
Uniprot ID | P08151 |
Protein Accession # | NP_005260 |
Nucleotide Accession # | NM_005269 |
Protein Size (# AA) | 1106 |
Molecular Weight | 118kDa |
Protein Interactions | TRIM42; GCC1; PRKAA2; VHL; FEM1B; CDK4; UBC; MYC; ITCH; NUMB; SUFU; RAI1; XBP1; HDAC2; HDAC1; STK36; DYRK1A; ZIC2; ZIC1; Sin3a; Shh; SAP18; Su(fu); Pias1; |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "GLI1 Antibody - C-terminal region : FITC (ARP32368_T100-FITC)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit".
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How long will it take to receive "GLI1 Antibody - C-terminal region : FITC (ARP32368_T100-FITC)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "GLI1 Antibody - C-terminal region : FITC (ARP32368_T100-FITC)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "GLI1 Antibody - C-terminal region : FITC (ARP32368_T100-FITC)"?
This target may also be called "GLI, PPD1, PAPA8" in publications.
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What is the shipping cost for "GLI1 Antibody - C-terminal region : FITC (ARP32368_T100-FITC)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "GLI1 Antibody - C-terminal region : FITC (ARP32368_T100-FITC)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "GLI1 Antibody - C-terminal region : FITC (ARP32368_T100-FITC)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "118kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "GLI1 Antibody - C-terminal region : FITC (ARP32368_T100-FITC)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "GLI1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "GLI1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "GLI1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "GLI1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "GLI1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "GLI1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.