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GLE1 Antibody (OAGA00556)

100 ul
$409.00
In Stock
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Gene Symbol:
GLE1
Official Gene Full Name:
Nucleoporin GLE1
NCBI Gene Id:
2733
Protein Name:
Nucleoporin GLE1
Swissprot Id:
Q53GS7
Protein Accession #:
NP_001003722.1
Alias Symbols:
GLE1L antibody, LCCS antibody, LCCS1 antibody, hGLE1 antibody, GLE1 antibody, "GLE1-like, RNA export mediator antibody", GLE1-like protein antibody, nucleoporin GLE1 antibody, GLE1 RNA export mediator homolog (yeast) antibody
Replacement Item:
This antibody may replace item sc-171697 from Santa Cruz Biotechnology.
Molecular Weight:
75 kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Purified by antigen-affinity chromatography.
Application:
ICC, IF, IHC-FFPE, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express GLE1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express GLE1.
Immunogen:
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 414 and 646 of GLE1
Predicted Species Reactivity:
Human
Product Format:
Liquid
Reconstitution and Storage:
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Concentration:
1 mg/ml
Datasheets/Manuals:
Printable datasheet for anti-GLE1 antibody - OAGA00556
Isotype:
IgG
Application Info:
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Additional Information:
Background: This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
:::
Positive Controls: 293T, A431, HeLa, Molt-4, Raji
:::
Cellular Localization: Nucleus, Cytoplasm, Isoform 1: Cytoplasm, nuclear pore complex
Storage Buffer:
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

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