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Catalog No: OKCD01916 (Formerly GWB-KBBOW7)
Size:96WELLS
Price: $675.00
SKU
OKCD01916
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Datasheets/ManualsPrintable datasheet for GLDC ELISA Kit (Human) (OKCD01916)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycine Dehydrogenase (GLDC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycine Dehydrogenase (GLDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycine Dehydrogenase (GLDC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glycine Dehydrogenase (GLDC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycine Dehydrogenase (GLDC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycine Dehydrogenase (GLDC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-GLDC Microplate96 Wells (12 x 8 Well Strips)
GLDC Lyophilized Standard2 x 20 ng
100X Biotinylated GLDC Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.132 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Glycine Dehydrogenase (GLDC). No significant cross-reactivity or interference between Glycine Dehydrogenase (GLDC) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolGLDC
Gene Full Nameglycine decarboxylase
Alias SymbolsGCE;GCSP;Glycine cleavage system P protein;glycine cleavage system protein P;Glycine decarboxylase;glycine decarboxylase P-protein;glycine dehydrogenase (aminomethyl-transferring);glycine dehydrogenase (decarboxylating), mitochondrial;glycine dehydrogenase [decarboxylating], mitochondrial;HYGN1;nonketotic hyperglycinemia.
NCBI Gene Id2731
Protein NameGlycine dehydrogenase (decarboxylating), mitochondrial
Description of TargetThe glycine cleavage system catalyzes the degradation of glycine. The P protein (GLDC) binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (GCSH).
Uniprot IDP23378
Protein Accession #NP_000161.2
Nucleotide Accession #NM_000170.2
Protein Size (# AA)1020
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