Catalog No: OKCD03533
Size:96WELLS
Price: $800.00
SKU
OKCD03533
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GKN2 Chemi-Luminescent ELISA Kit (Human) (OKCD03533)

Datasheets/ManualsPrintable datasheet for GKN2 Chemi-Luminescent ELISA Kit (Human) (OKCD03533)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Gastrokine 2 (GKN2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Gastrokine 2 (GKN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Gastrokine 2 (GKN2) level in the sample or standard.
ELISA Kit Range1.37-1,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gastrokine 2 (GKN2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gastrokine 2 (GKN2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
GKN2 Microplate96 Wells (12 x 8 Well strips)
GKN2 Lyophilized Standard2
100X GKN2 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity15.6 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGKN2
Gene Full Namegastrokine 2
Alias Symbolsblottin;BRICD1B;BRICHOS domain containing 1B;Down-regulated in gastric cancer;down-regulated in gastric cancer GDDR;gastrokine-2;GDDR;PRO813;TFIZ1;trefoil factor interactions(z) 1;VLTI465.
NCBI Gene Id200504
Protein NameGastrokine-2
Description of TargetGastrokine-2
Uniprot IDQ86XP6
Protein Accession #NP_872342
Nucleotide Accession #NM_182536
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