GJD2 ELISA Kit (Human) (OKEH07665)

Catalog No: OKEH07665

Size:96T

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology GJD2 ELISA Kit (Human) (OKEH07665) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GJD2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GJD2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GJD2 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.8% (n = 20)
  Mean Inter-assay CV%: <=8.1% (n = 20)
• ELISA Kit Component:
  • GJD2 Microplate: 96 Wells (12 x 8 Well strips)
  • GJD2 Lyophilized Standard: 2
  • 100X Biotinylated GJD2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
  Subcellular Location: Cell membrane Multi-pass membrane protein Cell junction Gap junction
  Unigene: Hs.283816
  SMR: Q9UKL4
  String: 9606.ENSP00000290374
  KEGG: hsa:57369
  MIM: 607058
  Pfam: PF00029
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Human Gap junction delta-2 protein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: GJD2
• Gene Full Name: gap junction protein delta 2
• Alias Symbols: connexin-36;CX36;gap junction alpha-9 protein;gap junction delta-2 protein;gap junction protein, delta 2, 36kDa;GJA9.
• NCBI Gene Id: 57369
• Protein Name: Q9UKL4
• Description of Target: Gap junction delta-2 protein
• Uniprot ID: https://www.uniprot.org/uniprot/Q9UKL4
• Protein Accession #: NP_065711.1
• Nucleotide Accession #: NM_020660.2