GJB2 Antibody (OABB00122)

Catalog No: OABB00122 (Formerly GWB-BBP045)

Size:100UG

WB: Rat Liver Tissue Lysate

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Hamster|Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Connexin26(CX26), also known as GAP junction protein, beta2, GJB2. Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. The 3-prime untranslated region of the CX26 transcript contains a putative mRNA instability sequence. The deduced 226-amino acid protein has a calculated molecular mass of about 26 kD. CX26 shares 92.5% identity with rat Cx26. connexin 26(GJB2) is assigned to human chromosome 13q11-q12 .Connexin 26 regulates epidermal barrier and wound remodeling and promotes psoriasiform response. Connexin 26 gene(GJB2) mutation modulates the severity of hearing loss associated with the 1555A-G mitochondrial mutation.
  Background: Connexin26(CX26), also known as GAP junction protein, beta2, GJB2. Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. The 3-prime untranslated region of the CX26 transcript contains a putative mRNA instability sequence. The deduced 226-amino acid protein has a calculated molecular mass of about 26 kD. CX26 shares 92.5% identity with rat Cx26. connexin 26 (GJB2) is assigned to human chromosome 13q11-q12 .Connexin 26 regulates epidermal barrier and wound remodeling and promotes psoriasiform response. Connexin 26 gene (GJB2) mutation modulates the severity of hearing loss associated with the 1555A-G mitochondrial mutation.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Rat: Mouse
• Reference: 1. Mignon, C.; Fromaget, C.; Mattei, M.-G.; Gros, D.; Yamasaki, H.; Mesnil, M. : Assignment of
  connexin 26 (GJB2) and 46 (GJA3) genes to human chromosome 13q11-q12 and mouse
  chromosome 14D1-E1 by in situ hybridization. Cytogenet. Cell Genet. 72: 185-186, 1996.
  PubMed ID : 8978770
  2. Djalilian, A. R.; McGaughey, D.; Patel, S.; Seo, E. Y.; Yang, C.; Cheng, J.; Tomic, M.; Sinha, S.;
  Ishida-Yamamoto, A.; Segre, J. A. : Connexin 26 regulates epidermal barrier and wound remodeling
  and promotes psoriasiform response. J. Clin. Invest. 116: 1243-1253, 2006.
  3. Abe, S.; Kelley, P. M.; Kimberling, W. J.; Usami, S. : Connexin 26 gene (GJB2) mutation modulates
  the severity of hearing loss associated with the 1555A-G mitochondrial mutation. Am. J. Med. Genet.
  103: 334-338, 2001.
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Gap junction beta-2 protein(GJB2) detection. Tested with WB in Human;Mouse;Rat.

Target Info

• Gene Symbol: GJB2
• Gene Full Name: gap junction protein beta 2
• Alias Symbols: BAPS;connexin 26;Connexin-26;CX26;DFNA3;DFNA3A;DFNB1;DFNB1A;gap junction beta 2 proteinc;gap junction beta-2 protein;gap junction protein, beta 2, 26kDa;HID;KID;mutant gap junction beta 2 protein;mutant gap junction protein beta 2;NSRD1;PPK.
• NCBI Gene Id: 2706
• Protein Name: Gap junction beta-2 protein
• Description of Target: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
• Uniprot ID: P29033
• Protein Accession #: NP_003995.2
• Nucleotide Accession #: NM_004004.5
• Molecular Weight: 26215 MW