Catalog No: OCOA14889
Size:20UG
Price: $179.00
SKU
OCOA14889
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GJB1 Protein Lysate (OCOA14889)

Datasheets/ManualsPrintable datasheet for GJB1 Protein Lysate (OCOA14889)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionGJB1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolGJB1
Gene Full Namegap junction protein beta 1
Alias SymbolsCMTX;CMTX1;connexin-32;CX32;GAP junction 28 kDa liver protein;gap junction beta-1 protein;gap junction protein, beta 1, 32kDa.
NCBI Gene Id2705
Protein NameNM_001097642, NP_001091111, P08034
Description of TargetGap junction beta-1 protein, gap junction beta-1 protein [Homo sapiens], Homo sapiens gap junction protein beta 1 (GJB1), transcript variant 1, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/P08034
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_001091111
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_001097642
Molecular Weight31.8 kDa
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