Catalog No: OKCD00872 (Formerly GWB-KBBMA8)
Size:96WELLS
Price: $675.00
SKU
OKCD00872
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Datasheets/ManualsPrintable datasheet for GJB1 ELISA Kit (Human) (OKCD00872)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gap Junction Protein Beta 1 (GJb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gap Junction Protein Beta 1 (GJb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gap Junction Protein Beta 1 (GJb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Gap Junction Protein Beta 1 (GJb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gap Junction Protein Beta 1 (GJb1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gap Junction Protein Beta 1 (GJb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-GJB1 Microplate96 Wells (12 x 8 Well Strips)
GJB1 Lyophilized Standard2 x 20 ng
100X Biotinylated GJB1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.108 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Gap Junction Protein Beta 1 (GJb1). No significant cross-reactivity or interference between Gap Junction Protein Beta 1 (GJb1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolGJB1
Gene Full Namegap junction protein beta 1
Alias SymbolsCMTX;CMTX1;connexin-32;CX32;GAP junction 28 kDa liver protein;gap junction beta-1 protein;gap junction protein, beta 1, 32kDa.
NCBI Gene Id2705
Protein NameGap junction beta-1 protein
Description of TargetOne gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Uniprot IDP08034
Protein Accession #NP_000157
Nucleotide Accession #NM_000166.5
Protein Size (# AA)283
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