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Datasheets/Manuals | Printable datasheet for anti-GJB1 (OAAD00146) antibody |
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Predicted Species Reactivity | Human, Mouse, Rat |
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Clonality | Monoclonal |
Clone | CXN-32 |
Isotype | IgG1 |
Host | Mouse |
Application | WB, IHC |
Reconstitution and Storage | At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time. |
Immunogen | Synthetic connexin-32 peptide (amino acids 105-123) conjugated to KLH. |
Purification | Purified by the goat anti-mouse IgG Affinity chromatography. |
Specificity | Human, mouse, rat. No cross reactivity with other proteins. |
Formulation | Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative. |
Application Info | Western blot At 0.5-1ug/ml with the appropriate system to detect connexin 32 in cells and tissues. Immunohistochemistry(F) At 1-2ug/ml to detect connexin 32 in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user. |
Storage Buffer | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml. |
Reference | 1. Corcos, I. A.; Lafreniere, R. G.; Begy, C. R.; Loch-Caruso, R.; Willard, H. F.; Glover, T. W. : Refined localization of human connexin 32 gene locus, GJB1, to Xq13.1. Genomics 13: 479-480, 1992. 2 Bondurand, N.; Girard, M.; Pingault, V.; Lemort, N.; Dubourg, O.; Goossens, M. : Human connexin 32, a gap junction protein altered in the X-linked form of Charcot-Marie-Tooth disease, is directly regulated by the transcription factor SOX10. Hum. Molec. Genet. 10: 2783-2795, 2001. 3. Fairweather, N.; Bell, C.; Cochrane, S.; Chelly, J.; Wang, S.; Mostacciuolo, M. L.; Monaco, A. P.; Haites, N. E. : Mutations in the connexin 32 gene in X-linked dominant Charcot-Marie-Tooth disease (CMTX1). Hum. Molec. Genet. 3: 29-34, 1994. |
Gene Symbol | GJB1 |
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Gene Full Name | Gap junction beta-1 protein |
Alias Symbols | CMTX, CX32, CMTX1 |
NCBI Gene Id | 2705 |
Protein Name | Gap junction beta-1 protein |
Description of Target | Connexin32(CX32), also known as GAP junction protein beta-1(GJB1). Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. CX32 expressed in liver and peripheral nerve. CX32 gene is mapped to Xp11-q13 and is directly regulated by the transcription factor SOX10. |
Uniprot ID | P08034 |
Protein Accession # | NP_000157.1; NP_001091111.1 |
Nucleotide Accession # | NM_000166.5; NM_001097642.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "GJB1 Antibody (OAAD00146)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
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How long will it take to receive "GJB1 Antibody (OAAD00146)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "GJB1 Antibody (OAAD00146)" provided in?
This item is provided in "".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "GJB1 Antibody (OAAD00146)"?
This target may also be called "CMTX, CX32, CMTX1" in publications.
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What is the shipping cost for "GJB1 Antibody (OAAD00146)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "GJB1 Antibody (OAAD00146)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "GJB1 Antibody (OAAD00146)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "GJB1 Antibody (OAAD00146)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "GJB1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "GJB1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "GJB1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "GJB1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "GJB1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "GJB1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.