GJB1 Antibody (OAAD00146)

Catalog No: OAAD00146

Size:100 ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone: CXN-32
• Isotype: IgG1
• Host: Mouse
• Application: WB, IHC
• Reconstitution and Storage: At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time.
• Immunogen: Synthetic connexin-32 peptide (amino acids 105-123) conjugated to KLH.
• Purification: Purified by the goat anti-mouse IgG Affinity chromatography.
• Specificity: Human, mouse, rat.
  No cross reactivity with other proteins.
• Formulation: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative.
• Application Info: Western blot At 0.5-1ug/ml with the appropriate system to detect connexin 32 in cells and tissues.
  Immunohistochemistry(F) At 1-2ug/ml to detect connexin 32 in formalin or acetone fixed tissues.
  Other applications have not been tested.
  Optimal dilutions should be determined by end user.
• Storage Buffer: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
• Reference: 1. Corcos, I. A.; Lafreniere, R. G.; Begy, C. R.; Loch-Caruso, R.; Willard, H. F.; Glover, T. W. : Refined localization of human connexin 32 gene locus, GJB1, to Xq13.1. Genomics 13: 479-480, 1992.
  2 Bondurand, N.; Girard, M.; Pingault, V.; Lemort, N.; Dubourg, O.; Goossens, M. : Human connexin 32, a gap junction protein altered in the X-linked form of Charcot-Marie-Tooth disease, is directly regulated by the transcription factor SOX10. Hum. Molec. Genet. 10: 2783-2795, 2001.
  3. Fairweather, N.; Bell, C.; Cochrane, S.; Chelly, J.; Wang, S.; Mostacciuolo, M. L.; Monaco, A. P.; Haites, N. E. : Mutations in the connexin 32 gene in X-linked dominant Charcot-Marie-Tooth disease (CMTX1). Hum. Molec. Genet. 3: 29-34, 1994.

Target Info

• Gene Symbol: GJB1
• Gene Full Name: Gap junction beta-1 protein
• Alias Symbols: CMTX, CX32, CMTX1
• NCBI Gene Id: 2705
• Protein Name: Gap junction beta-1 protein
• Description of Target: Connexin32(CX32), also known as GAP junction protein beta-1(GJB1). Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. CX32 expressed in liver and peripheral nerve. CX32 gene is mapped to Xp11-q13 and is directly regulated by the transcription factor SOX10.
• Uniprot ID: P08034
• Protein Accession #: NP_000157.1; NP_001091111.1
• Nucleotide Accession #: NM_000166.5; NM_001097642.2