GJA4 Antibody - N-terminal region (OABB01369)

Catalog No: OABB01369

Size:100ug

Anti-Connexin 37/GJA4 antibody, OABB01369, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: NIH3T3 Cell Lysate
Lane 5: SKOV Cell Lysate
Lane 6: A431 Cell Lysate

Anti-Connexin 37/GJA4 antibody, OABB01369, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

Anti-Connexin 37/GJA4 antibody, OABB01369, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue

Anti-Connexin 37/GJA4 antibody, OABB01369, ICC
ICC: HELA Cell

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Product Format: Lyophilized
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: WB, IHC-P, ICC
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Gap junction alpha-4 protein(GJA4), also called CX37 is a protein that in humans is encoded by the GJA4 gene. This gene is mapped to 1p34.3. This gene encodes a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene have been associated with atherosclerosis and a higher risk of myocardial infarction. CX37 had been shown to reflect the stage and maturity of luteinized follicles in animal studies.
• Reconstitution and Storage: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.. At -20C for one year from date of receipt. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for six months. Avoid repeated freezing and thawing.
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Connexin 37(3-17aa DWGFLEKLLDQVQEH), identical to the related mouse and rat sequences.
• Purification: Immunogen affinity purified.
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: Mouse: By Heat
  Immunocytochemistry : 0.5-1 ug/ml: Human: Mouse, Rat
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Gap junction alpha-4 protein(GJA4) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

Target Info

• Gene Symbol: GJA4
• Gene Full Name: gap junction protein, alpha 4, 37kDa
• Alias Symbols: Connexin 37, Connexin-37, Cx37, CXA4_HUMAN, Gap junction alpha 4 protein, Gap junction alpha-4 protein, gap junction protein, alpha 4 37kDa(connexin 37), gap junction protein, alpha 4, 37kD(connexin 37), Gap junction protein, alpha 4, 37kDa, Gja4, OTTHUMP00000004181, OTTHUMP00000004182
• Protein Name: Gap junction alpha-4 protein
• Description of Target: Gap junction alpha-4 protein(GJA4), also called CX37 is a protein that in humans is encoded by the GJA4 gene. This gene is mapped to 1p34.3. This gene encodes a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene have been associated with atherosclerosis and a higher risk of myocardial infarction. CX37 had been shown to reflect the stage and maturity of luteinized follicles in animal studies.
• Uniprot ID: P35212