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Catalog No: OKEH08503
Size:96T
Price: $800.00
SKU
OKEH08503
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPorcine, Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Linearity
Sample1:21:41:81:16
serum(n=5)104-114% (n = 20)91-101% (n = 20)110-119% (n = 20)83-94% (n = 20)
EDTA plasma(n=5)95-107% (n = 20)92-102% (n = 20)85-95% (n = 20)97-109% (n = 20)
heparin plasma(n=5)103-113% (n = 20)85-94% (n = 20)103-113% (n = 20)85-97% (n = 20)
ELISA Kit PrincipleAviva Systems Biology GJA1 ELISA Kit (Pig) (OKEH08503) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GJA1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GJA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GJA1 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 93% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=7.7% (n = 20)
Mean Inter-assay CV%: <=10.5% (n = 20)
ELISA Kit Component
ComponentAmount
GJA1 Microplate96 Wells (12 x 8 Well strips)
GJA1 Lyophilized Standard2
100X Biotinylated GJA1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract. May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles.
::Subcellular Location: Cell membrane Multi-pass membrane protein Cell junction Gap junction Endoplasmic reticulum Localizes at the intercalated disk (ICD) in cardiomyocytes and proper localization at ICD is dependent on TMEM65.
::Unigene: Ssc.1357
SMR: Q29101
String: 9823.ENSSSCP00000004580
::Pfam: PF00029
Reconstitution and StorageStore as indicated in product manual.
Sensitivity36pg/mL
SpecificityNatural and recombinant Pig Gap junction alpha-1 protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGJA1
Alias SymbolsConnexin-43.
Protein NameQ29101
Description of TargetGap junction alpha-1 protein
Uniprot IDhttps://www.uniprot.org/uniprot/Q29101
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