Catalog No: OKEH03884
Size:96T
Price: $625.00
SKU
OKEH03884
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Predicted Species Reactivity | Porcine, Sus scrofa | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology GIP ELISA Kit (Pig) (OKEH03884) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-pig GIP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GIP and incubated. The GIP found in the sample or standards competes with the biotinylated GIP for limited binding sites on the immobilized anti-pig GIP antibody. Excess unbound biotinylated GIP and sample or standard GIP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GIP captured in the well and inversely proportional to the amount of GIP which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 78-5000pg/mL | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
Sensitivity | 30 pg/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Pig Gastric inhibitory polypeptide | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA |
Gene Symbol | GIP |
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Gene Full Name | gastric inhibitory polypeptide |
Alias Symbols | Glucose-dependent insulinotropic polypeptide. |
NCBI Gene Id | 100533199 |
Protein Name | P01281 |
Description of Target | Gastric inhibitory polypeptide |
Uniprot ID | https://www.uniprot.org/uniprot/P01281 |
Protein Size (# AA) | 42 |
Molecular Weight | 5 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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