GHRL ELISA Kit (Cattle) (OKCD02570)

Catalog No: OKCD02570

Size:96WELLS

GHRL ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Bos taurus|Bovine
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 2h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	82-102%	88-102%	82-101%	80-90%
  EDTA Plasma (n=5)	88-97%	97-105%	86-103%	79-91%
  Heparin Plasma (n=5)	78-91%	80-88%	79-96%	78-101%

• ELISA Kit Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ghrelin (GHRL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ghrelin (GHRL) and unlabeled Ghrelin (GHRL) (Standards or samples) with the pre-coated antibody specific to Ghrelin (GHRL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ghrelin (GHRL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Ghrelin (GHRL) in the sample.
• ELISA Kit Range: 123.5-10,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	90-104	98
  EDTA Plasma (n=5)	96-103	101
  Heparin Plasma (n=5)	80-97	84

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ghrelin (GHRL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ghrelin (GHRL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Copy From Booklet: Copy From Booklet
  • Copy From Booklet: Copy From Booklet
  • Copy From Booklet: Copy From Booklet
  • Copy From Booklet: Copy From Booklet
  • Copy From Booklet: Copy From Booklet
  • Copy From Booklet: Copy From Booklet
  • Copy From Booklet: Copy From Booklet
  • Copy From Booklet: Copy From Booklet
  • Copy From Booklet: Copy From Booklet
  • Copy From Booklet: Copy From Booklet
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 49.2 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ghrelin (GHRL). No significant cross-reactivity or interference between Ghrelin (GHRL) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Competitive Inhibition ELISA

Target Info

• Gene Symbol: GHRL
• Gene Full Name: ghrelin and obestatin prepropeptide
• Alias Symbols: appetite-regulating hormone;Gh1-1;ghrelin;ghrelin/obestatin preprohormone;growth hormone secretagogue;growth hormone-releasing peptide;motilin-related peptide.
• NCBI Gene Id: 281192
• Protein Name: Appetite-regulating hormone
• Description of Target: Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation (By similarity).
• Uniprot ID: Q9BDJ6
• Protein Accession #: NP_776492.1
• Nucleotide Accession #: NM_174067.2
• Molecular Weight: 116 kDa