GHRHR ELISA Kit (Mouse) (OKCA01682)

Catalog No: OKCA01682

Size:96 Wells

Mouse GHRHR ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	98	89-101
  1:2	94	90-98
  1:4	103	95-107
  1:8	102	93-106

• ELISA Kit Principle: Aviva Systems Biology Ghrhr ELISA Kit (Mouse) (OKCA01682) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ghrhr has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ghrhr is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Ghrhr captured in the well.
• ELISA Kit Range: 39.06 - 2,500 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	104	95-108
  EDTA Plasma (n=4)	102	92-105

• ELISA Kit Component:
  • Ghrhr Microplate: 96 Wells (12 x 8 Well strips)
  • Ghrhr Lyophilized Standard: 2 x 2.5 ng
  • 100X Biotinylated Ghrhr Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 9.77 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: GHRHR
• Gene Full Name: growth hormone releasing hormone receptor
• Alias Symbols: Ghr, lit, Ghrfr, little
• NCBI Gene Id: 14602
• Protein Name: Growth hormone-releasing hormone receptor
• Description of Target: Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion.
• Uniprot ID: P32082
• Protein Accession #: NP_001003685.2
• Nucleotide Accession #: NM_001003685.3