GHRH ELISA Kit (Pig) (OKCD02569)

Catalog No: OKCD02569

Size:96WELLS

GHRH ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Porcine|Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 2h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	99-105%	87-101%	91-99%	79-98%
  EDTA Plasma (n=5)	96-105%	89-103%	89-96%	80-99%
  Heparin Plasma (n=5)	96-104%	79-102%	99-105%	86-105%

• ELISA Kit Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Growth Hormone Releasing Hormone (GHRH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Growth Hormone Releasing Hormone (GHRH) and unlabeled Growth Hormone Releasing Hormone (GHRH) (Standards or samples) with the pre-coated antibody specific to Growth Hormone Releasing Hormone (GHRH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Growth Hormone Releasing Hormone (GHRH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Growth Hormone Releasing Hormone (GHRH) in the sample.
• ELISA Kit Range: 12.35-1000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	89-103	93
  EDTA Plasma (n=5)	93-105	99
  Heparin Plasma (n=5)	85-102	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone Releasing Hormone (GHRH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone Releasing Hormone (GHRH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
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• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 5.22 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Growth Hormone Releasing Hormone (GHRH). No significant cross-reactivity or interference between Growth Hormone Releasing Hormone (GHRH) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Competitive Inhibition ELISA

Target Info

• Gene Symbol: GHRH
• Gene Full Name: growth hormone releasing hormone
• Alias Symbols: GRF;Growth hormone-releasing factor;Growth hormone-releasing hormone;somatoliberin.
• NCBI Gene Id: 100499556
• Protein Name: Somatoliberin
• Description of Target: GRF is released by the hypothalamus and acts on the adenohypophyse to stimulate the secretion of growth hormone.
• Uniprot ID: P01287
• Protein Accession #: NP_001182047.1
• Nucleotide Accession #: NM_001195118.1
• Molecular Weight: 44 kDa