GH2 Chemi-Luminescent ELISA Kit (Human) (OKCD03532)

Catalog No: OKCD03532

Size:96WELLS

Human GH2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	86-93%	81-90%	85-99%	86-104%
  EDTA Plasma (n=5)	91-103%	90-97%	94-103%	90-97%
  Heparin Plasma (n=5)	87-94%	98-105%	97-105%	78-88%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Growth Hormone 2 (GH2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Growth Hormone 2 (GH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Growth Hormone 2 (GH2) level in the sample or standard.
• ELISA Kit Range: 6.86-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	86-98	94
  EDTA Plasma (n=5)	87-105	101
  Heparin Plasma (n=5)	90-98	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone 2 (GH2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone 2 (GH2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • GH2 Microplate: 96 Wells (12 x 8 Well strips)
  • GH2 Lyophilized Standard: 2
  • 100X GH2 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.078 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: GH2
• Gene Full Name: growth hormone 2
• Alias Symbols: GHB2;GHL;GHV;GH-V;Growth hormone 2;growth hormone B2;growth hormone variant;hGH-V;placental-specific growth hormone;placenta-specific growth hormone.
• NCBI Gene Id: 2689
• Protein Name: Growth hormone variant
• Description of Target: Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
• Uniprot ID: P01242
• Protein Accession #: NP_002050
• Nucleotide Accession #: NM_002059