Catalog No: OKEH02966
Size:96T
Price: $425.00
SKU
OKEH02966
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology GH1 ELISA Kit (Rat) (OKEH02966) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-rat Gh1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Gh1 and incubated. The Gh1 found in the sample or standards competes with the biotinylated Gh1 for limited binding sites on the immobilized anti-rat Gh1 antibody. Excess unbound biotinylated Gh1 and sample or standard Gh1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Gh1 captured in the well and inversely proportional to the amount of Gh1 which was contained in the sample or standard.
ELISA Kit Range78-5000pg/mL
ELISA Kit Component
ComponentAmount
Anti-Gh1 Microplate96 Wells (12 x 8 Well strips)
Gh1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Gh1-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity39.7 pg/mL
SpecificityNatural and recombinant Rat Somatotropin
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolGh1
Gene Full Namegrowth hormone 1
Alias SymbolsGh;Ghb1;Growth hormone;growth hormone b1;RNGHGP;somatotropin.
NCBI Gene Id24391
Protein NameP01244
Description of TargetSomatotropin
Uniprot IDhttps://www.uniprot.org/uniprot/P01244
Protein Accession #NP_001030020.2
Nucleotide Accession #NM_001034848.2
Protein Size (# AA)216
Molecular Weight25 kDa
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