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Catalog No: OKCA00874
Size:96 Wells
Price: $645.00
SKU
OKCA00874
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMonkey
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
ELISA Kit PrincipleAviva Systems Biology GH1 ELISA Kit (Monkey) (OKCA00874) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for GH1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GH1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample GH1 captured in well.
ELISA Kit Range2.5 ng/mL-50 ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level GH1 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level GH1 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
GH1 Microplate96 Wells (12 x 8 Well strips)
GH1 Lyophilized Standard2
100X Biotinylated GH1 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma
Sensitivity1 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolGH1
Gene Full NameSomatotropin
Alias SymbolsGHB3
NCBI Gene Id718156
Protein NameSomatotropin
Description of TargetPlays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
Uniprot IDP33093
Protein Accession #NP_001036203.1
Nucleotide Accession #NM_001042738.1
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