GGT5 ELISA Kit (Human) (OKCD00692)

Catalog No: OKCD00692 (Formerly GWB-KBBIQ2)

Size:96WELLS

Human GGT5 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	93-104%	95-104%	86-95%	79-102%
  EDTA plasma(n=5)	79-94%	79-101%	85-104%	94-102%
  heparin plasma(n=5)	85-92%	94-101%	82-96%	81-99%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gamma-Glutamyltransferase 5 (gGT5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gamma-Glutamyltransferase 5 (gGT5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gamma-Glutamyltransferase 5 (gGT5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Gamma-Glutamyltransferase 5 (gGT5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	78-96	90
  EDTA plasma(n=5)	79-99	78
  heparin plasma(n=5)	78-89	81

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gamma-Glutamyltransferase 5 (gGT5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gamma-Glutamyltransferase 5 (gGT5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-GGT5 Microplate: 96 Wells (12 x 8 Well Strips)
  • GGT5 Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated GGT5 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.55 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Gamma-Glutamyltransferase 5 (gGT5). No significant cross-reactivity or interference between Gamma-Glutamyltransferase 5 (gGT5) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: GGT5
• Gene Full Name: gamma-glutamyltransferase 5
• Alias Symbols: gamma-glutamyl cleaving enzyme;gamma-glutamyl transpeptidase-related enzyme;gamma-glutamyl transpeptidase-related protein;Gamma-glutamyltransferase 5;gamma-glutamyltransferase-like activity 1;gamma-glutamyltranspeptidase 5;GGL;GGT 5;GGTLA1;GGT-REL;glutathione hydrolase 5 proenzyme;leukotriene-C4 hydrolase.
• NCBI Gene Id: 2687
• Protein Name: Glutathione hydrolase 5 proenzyme
• Description of Target: Cleaves the gamma-glutamyl peptide bond of glutathione conjugates, but maybe not glutathione itself. Converts leukotriene C4 (LTC4) to leukotriene D4 (LTD4).
• Uniprot ID: P36269
• Protein Accession #: NP_001093251
• Nucleotide Accession #: NM_001099781.2
• Protein Size (# AA): 586