GGT1 ELISA Kit (Human) (OKCD07368)

Catalog No: OKCD07368

Size:96WELLS

GGT1 ELISA Kit (Human) (OKCD07368) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gamma-Glutamyltransferase 1 (gGT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gamma-Glutamyltransferase 1 (gGT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gamma-Glutamyltransferase 1 (gGT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Gamma-Glutamyltransferase 1 (gGT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gamma-Glutamyltransferase 1 (gGT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gamma-Glutamyltransferase 1 (gGT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • GGT1 Microplate: 96 Wells (12 x 8 Well strips)
  • GGT1 Lyophilized Standard: 2
  • 100X GGT1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.59ng/mL

Target Info

• Gene Symbol: GGT1
• Gene Full Name: gamma-glutamyltransferase 1
• Alias Symbols: CD224;D22S672;D22S732;Gamma-glutamyltransferase 1;gamma-glutamyltranspeptidase 1;GGT;GGT 1;GGTD;glutathione hydrolase 1 proenzyme;GTG;leukotriene-C4 hydrolase;testicular tissue protein Li 73.
• NCBI Gene Id: 2678
• Protein Name: Glutathione hydrolase 1 proenzyme
• Description of Target: Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates, and other gamma-glutamyl compounds. The metabolism of glutathione releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Isoform 3 seems to be inactive.
• Uniprot ID: P19440
• Protein Accession #: NP_001275762.1
• Nucleotide Accession #: NM_001288833.1