GGCX ELISA Kit (Human) (OKEH08326)

Catalog No: OKEH08326

Size:96T

Human GGCX ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology GGCX ELISA Kit (Human) (OKEH08326) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GGCX has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GGCX is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GGCX captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.4% (n = 20)
  Mean Inter-assay CV%: <=9.3% (n = 20)
• ELISA Kit Component:
  • GGCX Microplate: 96 Wells (12 x 8 Well strips)
  • GGCX Lyophilized Standard: 2
  • 100X Biotinylated GGCX Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Mediates the vitamin K-dependent carboxylation of glutamate residues to calcium-binding gamma-carboxyglutamate (Gla) residues with the concomitant conversion of the reduced hydroquinone form of vitamin K to vitamin K epoxide.
  Subcellular Location: Endoplasmic reticulum membrane Multi-pass membrane protein
  Unigene: Hs.77719
  SMR: P38435
  String: 9606.ENSP00000233838
  KEGG: hsa:2677
  MIM: 137167
  Pfam: PF05090
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 39.9pg/mL
• Specificity: Natural and recombinant Human Vitamin K-dependent gamma-carboxylase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: GGCX
• Gene Full Name: gamma-glutamyl carboxylase
• Alias Symbols: Gamma-glutamyl carboxylase;peptidyl-glutamate 4-carboxylase;Vitamin K gamma glutamyl carboxylase;vitamin K-dependent gamma-carboxylase;VKCFD1.
• NCBI Gene Id: 2677
• Protein Name: P38435
• Description of Target: Vitamin K-dependent gamma-carboxylase
• Uniprot ID: https://www.uniprot.org/uniprot/P38435
• Protein Accession #: NP_000812.2
• Nucleotide Accession #: NM_000821.6