Catalog No: OKCD09312
Size:96WELLS
Price: $750.00
SKU
OKCD09312
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Printable datasheet for GFRA1 ELISA Kit (Human) (OKCD09312) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 0.119ng/mL |
Gene Symbol | GFRA1 |
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Gene Full Name | GDNF family receptor alpha 1 |
Alias Symbols | GDNF family receptor alpha-1;GDNF receptor alpha 1d;GDNF receptor alpha 1e;GDNFR;GDNFRA;GDNFR-alpha-1;GFRalpha-1;GFR-ALPHA-1;Glial cell line-derived neurotrophic factor receptor alpha;GPI-linked anchor protein;PI-linked cell-surface accessory protein;RET ligand 1;RET1L;RETL1;TGF-beta-related neurotrophic factor receptor 1;TRNR1. |
NCBI Gene Id | 2674 |
Protein Name | GDNF family receptor alpha-1 |
Description of Target | Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor (By similarity). |
Uniprot ID | P56159 |
Protein Accession # | NP_001138925.1 |
Nucleotide Accession # | NM_001145453.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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