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- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for GFP Antibody (OARA01906) |
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Predicted Species Reactivity | Green Fluorescent Protein |
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Product Format | Lyophilized |
Clonality | Monoclonal |
Clone | 9F9.F9 |
Host | Mouse |
Conjugation | HRP |
Application | Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot |
Additional Information | Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Color: Substrate dependent Conjugation Reference: Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. |
:: | Label: Peroxidase (Horseradish) Preservative: 0.01% (w/v) Thimerisol Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Reconstitution and Storage | 2°C to 8°C|-20°C or -80°C |
Immunogen | Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. |
Predicted Homology Based on Immunogen Sequence | wt, rGFP, eGFP |
Concentration | 1 mg/ml |
Application Info | Monclonal anti-GFP is designed to detect native GFP and GFP containing recombinant proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with Aviva's polyclonal anti-GFP (600-101-215) using either form of the antibody as the capture or detection antibodies. Only use the monoclonal form for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP. While not yet tested, this antibody will likely detect GFP and GFP containing proteins by immunofluorescence microscopy, with properties similar to its companion polyclonal antibody. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. |
Gene Symbol | GFP |
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Gene Full Name | Green fluorescent protein |
Protein Name | Green fluorescent protein |
Description of Target | Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. |
Uniprot ID | P42212 |
Molecular Weight | 40000 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "GFP Antibody (OARA01906)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Green Fluorescent Protein".
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How long will it take to receive "GFP Antibody (OARA01906)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "GFP Antibody (OARA01906)" provided in?
This item is provided in "Lyophilized".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "GFP Antibody (OARA01906)"?
This target may also be called "" in publications.
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What is the shipping cost for "GFP Antibody (OARA01906)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "GFP Antibody (OARA01906)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "GFP Antibody (OARA01906)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "40000".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "GFP Antibody (OARA01906)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "GFP"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "GFP"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "GFP"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "GFP"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "GFP"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "GFP"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.