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GFP Antibody (OAEA00007)

  • Catalog#: OAEA00007
  • Domestic: within 3-5 day for delivery. International: 1-2 business weeks
50ug
$49.00
In Stock
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Gene Symbol:
GFP
Official Gene Full Name:
Green fluorescent protein
Protein Name:
Green fluorescent protein
Swissprot Id:
P42212
Host:
Mouse
Clonality:
Monoclonal
Application:
WB
Immunogen:
GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates
Product Format:
50 ug at 1mg/mL in 10mM PBS, pH 7.2
Reconstitution and Storage:
Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store at 4C (add 0.1% NaN3) for several days to weeks, and at -20C for longer periods. 
Datasheets/Manuals:
Printable datasheet for anti-GFP (OAEA00007) antibody
Specificity:
Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP
Clone:
GF28R
Purity:
Protein A affinity chromatography from mouse ascites fluid
Isotype:
IgG1
Additional Information:
React: Dot, ELISA, IP, IS, WB
:::
Sensitivity: WB (with ECL): 1-10ng of purified GFP or GFP fusion proteins
Publications:

Yale, K; Tackett, AJ; Neuman, M; Bulley, E; Chait, BT; Wiley, E; Phosphorylation-Dependent Targeting of Tetrahymena HP1 to Condensed Chromatin. 1, (2016). WB, 27579368

Arthur, LL; Chung, JJ; Jankirama, P; Keefer, KM; Kolotilin, I; Djuranovic, SP; Chalker, DL; Grbic, V; Green, R; Menassa, R; True, HL; Skeath, JB; Djuranovic, S; Rapid generation of hypomorphic mutations. , (2017). WB, 28106166

Protocol Information:
WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

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