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Catalog No: OCOA18834
Size:20UG
Price: $169.00
SKU
OCOA18834
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GFI1B Protein Lysate (OCOA18834)

Datasheets/ManualsPrintable datasheet for GFI1B Protein Lysate (OCOA18834)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionGFI1B HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolGFI1B
Gene Full Namegrowth factor independent 1B transcriptional repressor
Alias SymbolsBDPLT17;growth factor independent 1B (potential regulator of CDKN1A, translocated in CML);growth factor independent 1B transcription repressor;Growth factor independent protein 1B;Potential regulator of CDKN1A translocated in CML;zinc finger protein Gfi-1b;ZNF163B.
NCBI Gene Id8328
Protein NameNM_001135031, NP_001128503, Q5VTD9
Description of TargetHomo sapiens growth factor independent 1B transcriptional repressor (GFI1B), transcript variant 2, mRNA, Zinc finger protein Gfi-1b, zinc finger protein Gfi-1b isoform 2 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q5VTD9
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_001128503
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_001135031
Molecular Weight32.2 kDa
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