GFAP Recombinant Antibody [6B12] (OACA12092)

Catalog No: OACA12092

Size:100UL

Overlay Peak curve showing Jurkat cells stained with OACA12092 (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunofluorescence staining of SH-SY5Y cell with OACA12092 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescence staining of U87 cell with OACA12092 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

IHC image of OACA12092 diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of OACA12092 diluted at 1:300 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Positive WB detected in: MCF7 whole cell lysate,Mouse Brain tissue lysate,Rat Brain tissue lysate
All lanes: GFAP antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 50 kDa
Observed band size: 55 kDa
" " data-caption=""Western Blot
Positive WB detected in: MCF7 whole cell lysate,Mouse Brain tissue lysate,Rat Brain tissue lysate
All lanes: GFAP antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 50 kDa
Observed band size: 55 kDa
" " data-size="195x231" class="pxp-cover">"Western Blot
Positive WB detected in: MCF7 whole cell lysate,Mouse Brain tissue lysate,Rat Brain tissue lysate
All lanes: GFAP antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 50 kDa
Observed band size: 55 kDa
"

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Recombinant Monoclonal
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay,
  Flow cytometry,
  Immunofluorescence,
  Immunohistochemistry,
  Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: A synthesized peptide derived from Human GFAP
• Purification: Affinity Purified

Target Info

• Gene Symbol: GFAP
• Gene Full Name: glial fibrillary acidic protein
• Alias Symbols: ALXDRD;glial fibrillary acidic protein.
• NCBI Gene Id: 2670
• Protein Name: Glial fibrillary acidic protein
• Description of Target: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
• Uniprot ID: P14136