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GFAP Antibody (OASA00321)

0.1 mg
$329.00
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Gene Symbol:
GFAP
NCBI Gene Id:
24387
Protein Name:
Glial fibrillary acidic protein
Swissprot Id:
P14136
Protein Accession #:
NP_001124491.1
Alias Symbols:
FLJ45472, GFAP
Replacement Item:
This antibody may replace item sc-115582 from Santa Cruz Biotechnology.
Description of Target:
MOUSE ANTI BOVINE GFAP
Protein Size (# AA):
431
Host:
Mouse
Clonality:
Monoclonal
Application:
IHC-AFF, IHC-FFPE, ELISA, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express GFAP.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express GFAP.
Immunogen:
The immunogen for anti-GFAP antibody: bovine spinal cord homogenate.
Predicted Species Reactivity:
Cow
Predicted Homology Based on Immunogen Sequence:
Cow
Product Format:
Purified IgG - liquid
Reconstitution and Storage:
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-GFAP antibody - OASA00321
Specificity:
GFAP
Clone:
1B4
Isotype:
IgG2b
Application Info:
Immunohistology - Paraffin(1):    1/200 - 1/1000
(1) Special conditions apply - see [*]
Application Data:
Application #1: Staining of paraffin embedded human brain with Mouse anti Bovine GFAP following pronase treatment
Additional Information:
Histology Positive Control Tissue: Brain
:::
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% Sodium Azide (NaN3)
:::
Approx Protein Conc: IgG concentration 1.0mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Target Reference:
1. Pegram, C.N. et al. (1985) Monoclonal antibodies reactive with epitopes restricted to glial fibrillary acidic proteins of several species. Neurochem. Pathol. 3: 119-138.
2. McLendon, R.E. et al. (1986) The immunohistochemical application of three anti-GFAP monoclonal antibodies to formalin-fixed, paraffin-embedded, normal and neoplastic brain tissues. J. Neuropathol. Exp. Neurol. 45: 692-703.

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