GEN1 Antibody - N-terminal region (OAAB06090)

Catalog No: OAAB06090

Size:400ul

Western blot analysis of GEN1 Antibody (N-term) in mouse liver tissue lysates (35ug/lane). GEN1 (arrow) was detected using the purified Pab.

All lanes : Anti-GEN1 Antibody (N-term) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 103 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysate from MDA-MB-453 cell line, using GEN1 Antibody (N-term)(Cat. #OAAB06090). OAAB06090 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB
  Function: Endonuclease which resolves Holliday junctions by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation.
  Cellular Location: Nucleus.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This GEN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 amino acids from the N-terminal region of human GEN1.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:2000
  WB: 1:1000

Target Info

• Gene Symbol: GEN1
• Alias Symbols: Gen
• NCBI Gene Id: 348654
• Protein Name: Flap endonuclease GEN homolog 1
• Description of Target: Endonuclease which cleaves flap structures at the junction between single-stranded DNA and double-stranded DNA. Specific for 5'-overhanging flap structures in which the 5'-upstream of the flap is completely double-stranded. Prefers the blocked-flap structures similar to those occurring at replication forks, in which the 5' single-strand overhang of the flap is double-stranded.
• Uniprot ID: Q17RS7
• Protein Accession #: NP_001123481.1
• Molecular Weight: 103 kDa